| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/ 20-100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PDXPO; HEL-S-302 |
| Entrez GeneID | 55163; |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic peptide corresponding to internal residues of human PNPO |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PNPO抗体的3篇参考文献的简要信息(注：以下内容为模拟示例，实际文献需通过学术数据库查询确认)：
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1. **文献名称**：*Pyridoxamine 5'-phosphate oxidase deficiency: A rare cause of neonatal epileptic encephalopathy*
**作者**：Mills PB, et al.
**摘要**：该研究报道了PNPO基因突变导致的维生素B6代谢紊乱，引发新生儿顽固性癫痫及脑病。通过患者样本分析，揭示了PNPO酶活性缺失与维生素B6辅助治疗反应的关系。
2. **文献名称**：*Characterization of human pyridoxine 5'-phosphate oxidase and its interaction with pyridoxal 5'-phosphate*
**作者**：Hahn SH, et al.
**摘要**：研究利用重组PNPO蛋白制备特异性抗体，分析了PNPO在不同组织中的表达及其与底物PLP的相互作用，为理解其代谢调控机制提供依据。
3. **文献名称**：*Antibody-based profiling of PNPO expression in murine brain development*
**作者**：Guerin A, et al.
**摘要**：通过免疫组化及Western blot技术，探究PNPO抗体在小鼠大脑发育中的定位表达，发现其与神经元成熟及突触功能的相关性，提示PNPO在神经发育中的关键作用。
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如需更准确的文献，建议在PubMed或Google Scholar中搜索关键词“PNPO antibody”“pyridoxine 5'-phosphate oxidase”或结合疾病关键词(如“epilepsy”“vitamin B6”)。
Pyridoxamine 5'-phosphate oxidase (PNPO) is a critical enzyme in vitamin B6 metabolism, responsible for catalyzing the conversion of pyridoxine 5'-phosphate and pyridoxamine 5'-phosphate into pyridoxal 5'-phosphate (PLP), the active coenzyme form essential for over 160 enzymatic reactions. These include neurotransmitter synthesis, amino acid metabolism, and hemoglobin production. PNPO deficiency, a rare autosomal recessive disorder caused by mutations in the PNPO gene, disrupts PLP synthesis, leading to severe neonatal-onset seizures resistant to conventional antiepileptics but responsive to PLP or pyridoxine supplementation.
PNPO antibodies are immunological tools developed to detect and quantify PNPO protein expression in research and diagnostics. They are widely used in techniques like Western blotting, immunohistochemistry, and ELISA to study PNPO's tissue distribution, expression patterns, and regulatory mechanisms. Such antibodies have been instrumental in elucidating PNPO's role in neurological health and disease, particularly in vitamin B6-dependent epilepsies. Additionally, they aid in diagnosing PNPO deficiency by identifying reduced enzyme levels or abnormal isoforms in patient samples. Both monoclonal and polyclonal PNPO antibodies are available, with applications ranging from basic research on metabolic pathways to clinical biomarker validation. Recent studies also explore PNPO's potential links to cancer and neurodegenerative disorders, expanding the utility of these antibodies in translational medicine.
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