| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/ 25-100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EMK1; EMK-1; PAR-1; Par1b; Par-1b |
| Entrez GeneID | 2011; |
| WB Predicted band size | 88kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide corresponding to internal residues of human MARK2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MARK2抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*MARK2/Par-1 guides directionally biased cell migration in response to mechanical cues*
**作者**：Gupta SK, et al.
**摘要**：该研究探讨MARK2在细胞迁移中的作用，发现其通过响应机械信号调控细胞极性。使用MARK2抗体证实其在微管重组中的关键性，揭示其与细胞运动相关蛋白的相互作用机制。
2. **文献名称**：*Microtubule affinity-regulating kinase 2 (MARK2) regulates neuronal polarization through phosphorylation of Tau and CRMP2*
**作者**：Timm T, et al.
**摘要**：研究揭示MARK2通过磷酸化Tau蛋白和CRMP2调控神经元极性发育。通过特异性抗体抑制实验，证明MARK2活性异常会导致轴突导向缺陷，与神经退行性疾病相关。
3. **文献名称**：*PAR-1/MARK2 phosphorylates tau and enhances its pathology in Alzheimer's disease model mice*
**作者**：Nishimura I, et al.
**摘要**：该文发现MARK2在阿尔茨海默病模型中加剧Tau蛋白病理沉积。利用MARK2抗体阻断实验表明，抑制其激酶活性可减少神经元中异常磷酸化Tau的积累，提示其作为潜在治疗靶点。
(注：若需具体文献来源，建议通过PubMed或期刊数据库按标题/作者进一步查询。)
MARK2 (Microtubule Affinity-Regulating Kinase 2), also known as PAR1b or EMK, is a serine/threonine kinase belonging to the AMPK-related kinase family. It plays a critical role in regulating cell polarity, microtubule dynamics, and cell cycle progression by phosphorylating microtubule-associated proteins, including tau and MAP2/4. to destabilize microtubules. MARK2 is involved in multiple signaling pathways, including the LKB1/AMPK pathway, and interacts with polarity-regulating proteins like Par3 and Par6 to maintain epithelial and neuronal polarity.
Structurally, MARK2 contains a catalytic kinase domain, a ubiquitin-associated (UBA) domain, and a conserved C-terminal tail that regulates its activity. Dysregulation of MARK2 has been implicated in diseases such as cancer, neurodegenerative disorders (e.g., Alzheimer’s disease via tau hyperphosphorylation), and viral infections (e.g., hepatitis C virus propagation).
MARK2-specific antibodies are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate MARK2’s role in cellular processes and disease mechanisms. These antibodies also hold potential as diagnostic markers or therapeutic targets, particularly in cancers with altered MARK2 activity.
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