| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ANOP3; MCOPS3 |
| Entrez GeneID | 6658; |
| WB Predicted band size | 34kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human SOX2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于SOX2抗体的3篇参考文献示例(虚构内容，仅供格式参考)：
---
1. **文献名称**: *SOX2 Antibody Validation in Embryonic Stem Cell Pluripotency Analysis*
**作者**: Smith J, et al.
**摘要**: 研究通过Western blot和免疫荧光验证了SOX2抗体在胚胎干细胞中的特异性，证明SOX2在维持多能性中的关键作用。
2. **文献名称**: *SOX2 as a Biomarker in Lung Cancer: Immunohistochemical Detection*
**作者**: Lee H, et al.
**摘要**: 利用SOX2抗体进行免疫组化分析，发现其在肺鳞癌中高表达，提示其作为诊断标志物的潜力。
3. **文献名称**: *SOX2 Antibody Application in Neural Progenitor Cell Studies*
**作者**: Chen R, et al.
**摘要**: 通过流式细胞术和染色质免疫沉淀(ChIP)，证实SOX2抗体可特异性标记神经前体细胞，支持其在神经再生研究中的应用。
---
(注：以上文献为示例，实际引用需检索真实数据库如PubMed。)建议通过关键词“SOX2 antibody validation”或“SOX2 immunohistochemistry”在学术平台查找具体文献。
The SOX2 antibody is a crucial tool in studying the SOX2 protein, a member of the SRY-related HMG-box (SOX) transcription factor family. SOX2 plays pivotal roles in maintaining pluripotency in embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), regulating self-renewal and differentiation. It is essential during early embryogenesis, particularly in neural development, eye formation, and establishing cell fate. Dysregulation of SOX2 is linked to developmental disorders (e.g., anophthalmia/microphthalmia) and cancers (e.g., lung, breast, and glioblastoma), where its overexpression often correlates with tumor aggressiveness and stem-like properties.
SOX2 antibodies are widely used in research to detect and quantify SOX2 expression via techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. They aid in characterizing stem cell populations, investigating tumor biology, and validating cellular reprogramming. Commercially available SOX2 antibodies vary in clonality (monoclonal/polyclonal), epitope specificity, and host species, requiring validation for target applications. Specificity is critical due to potential cross-reactivity with other SOX family members (e.g., SOX1. SOX3).
In clinical contexts, SOX2 antibodies serve as diagnostic markers for certain cancers and teratomas. Their utility extends to developmental biology studies, particularly in understanding SOX2’s interplay with OCT4. NANOG, and other pluripotency factors. Ongoing research continues to explore SOX2’s dual roles in regeneration and oncogenesis, underscoring the antibody’s importance in both basic and translational science.
×
