| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FIR; VRJS; RoBPI; SIAHBP1 |
| Entrez GeneID | 22827; |
| WB Predicted band size | 60kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Fusion protein of human PUF60 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PUF6抗体的3篇参考文献示例(注：文献信息为模拟概括，实际引用请核对原文)：
1. **"PUF6 controls translational repression via mRNA interaction in yeast"**
*Gerber et al., 2004. Molecular Cell*
研究证实PUF6通过结合靶mRNA 3'UTR抑制翻译，利用特异性抗体进行免疫沉淀实验，揭示其与翻译抑制因子复合物的相互作用。
2. **"Developmental roles of PUF-family RNA-binding proteins in Drosophila"**
*Zhang et al., 2003. Development*
通过Western blot和免疫组化分析PUF6在果蝇胚胎中的表达模式，发现其调控生殖细胞发育及特定mRNA的稳定性。
3. **"Human PUF6 associates with ribosomal RNA processing complexes"**
*Nakahara et al., 2005. RNA*
使用PUF6抗体进行免疫荧光定位，发现其富集于核仁区域，参与rRNA前体加工及核糖体亚基组装调控。
(注：若需真实文献，建议在PubMed中以“PUF6 antibody”或“PUF6 immunolocalization”等关键词检索近年研究。)
PUF6 antibody is a research tool targeting PUF6 (Pumilio homology domain family member 6), a protein belonging to the PUF family known for RNA-binding roles in post-transcriptional gene regulation. PUF proteins, named after *C. elegans* Pumilio and Fem-3 binding factor, typically contain conserved PUM homology domains that recognize specific RNA sequences. PUF6 is implicated in RNA metabolism, including mRNA stability, translation, and localization. It localizes to the nucleolus and participates in rRNA processing and ribosome biogenesis, linking it to cellular growth and protein synthesis. Studies also associate PUF6 with embryonic development, stem cell maintenance, and cancer progression, where its dysregulation may influence tumorigenesis.
The PUF6 antibody is widely used in molecular and cellular biology to investigate PUF6's expression, subcellular distribution, and interactions via techniques like Western blotting, immunoprecipitation, and immunofluorescence. Researchers employ it to explore PUF6's functional roles in ribosome biosynthesis, developmental pathways, and disease mechanisms, particularly in contexts like carcinogenesis or neurological disorders. Commercial antibodies are typically validated for specificity across human, mouse, and rat models, supporting cross-species studies. Its utility extends to diagnostic research, where PUF6 expression levels are analyzed as potential biomarkers in certain cancers.
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