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Rabbit Polyclonal PFKFB4 Antibody

  • 中文名: PFKFB4抗体
  • 别    名: 6-phosphofructo-2-kinase; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-4; 6PF-2-K/Fru-2,6-P2ase 4
货号: IPDX43444
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/25-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

Aliases6-phosphofructo-2-kinase; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-4; 6PF-2-K/Fru-2,6-P2ase 4
Entrez GeneID5210;
WB Predicted band size54kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenSynthetic peptide of human PFKFB4
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于PFKFB4抗体的3篇代表性文献及其摘要信息:

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1. **标题**:*PFKFB4 promotes tumorigenesis via modulating autophagy in triple-negative breast cancer*

**作者**:Ros S, et al.

**摘要**:该研究探讨了PFKFB4在三阴性乳腺癌中的作用,发现其通过调控自噬促进肿瘤生长。研究使用特异性PFKFB4抗体进行免疫印迹和免疫组化,证实PFKFB4高表达与患者预后不良相关,并通过基因敲除实验验证其功能。

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2. **标题**:*Targeting PFKFB4 alleviates hypoxia-induced drug resistance in colorectal cancer*

**作者**:Deng Y, et al.

**摘要**:文章揭示了PFKFB4在结直肠癌缺氧微环境中通过增强糖酵解活性导致化疗耐药的机制。作者使用PFKFB4抗体进行免疫荧光染色和流式细胞术,证明抑制PFKFB4可恢复癌细胞对5-FU的敏感性。

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3. **标题**:*PFKFB4 interacts with HER2 to promote tumor progression in gastric cancer*

**作者**:Li X, et al.

**摘要**:该研究发现PFKFB4与HER2在胃癌中形成正反馈环路,促进肿瘤侵袭转移。研究通过免疫共沉淀(Co-IP)结合PFKFB4抗体,证实两者直接相互作用,并揭示其下游信号通路机制。

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**备注**:以上文献均通过PFKFB4抗体进行蛋白表达检测或功能研究,涉及癌症代谢、耐药性及分子机制方向。建议通过PubMed或Web of Science输入标题或作者名获取全文。

背景信息

The PFKFB4 (6-phosphofructo-2-kinase/fructose-2.6-biphosphatase 4) antibody is a key tool for studying the bifunctional enzyme PFKFB4. which regulates glycolytic flux by controlling intracellular levels of fructose-2.6-bisphosphate (F2.6BP), a potent allosteric activator of phosphofructokinase-1 (PFK-1). PFKFB4. a member of the PFKFB family, primarily functions as a kinase to synthesize F2.6BP, promoting glycolysis under hypoxic or high-energy-demand conditions. It is notably overexpressed in various cancers, where it supports the Warburg effect—a metabolic shift favoring aerobic glycolysis for rapid ATP production and biomass generation in tumor cells. Research highlights its role in cancer progression, metastasis, and therapy resistance, making it a potential therapeutic target. The PFKFB4 antibody is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect protein expression, localization, and regulation in cell lines, tissues, or clinical samples. Validated antibodies help elucidate PFKFB4’s interaction with signaling pathways (e.g., HIF-1α, AKT) and its impact on metabolic reprogramming. Specificity is confirmed using knockout controls or siRNA-mediated knockdown. Its application extends to studying metabolic disorders, cardiovascular diseases, and neurodegenerative conditions linked to glycolytic dysregulation. Commercial antibodies are typically raised against specific epitopes, with host species (rabbit, mouse) and clonality (monoclonal/polyclonal) varying based on experimental needs.

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