| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | Protein SOX-15, Sox15, Sox-15 |
| Entrez GeneID | 20670 |
| WB Predicted band size | 25.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This (Mouse) Sox15 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 152-165 amino acids from the C-terminal region of human (Mouse) Sox15. |
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以下是关于SERCA1抗体的3篇参考文献示例(内容基于公开文献概括，具体文献可能需要根据实际研究补充)：
1. **文献名称**：*"Monoclonal antibodies against the Ca²⁺-ATPase of sarcoplasmic reticulum identify a protein restricted to fast-twitch muscles"*
**作者**：MacLennan DH, et al.
**摘要**：该研究报道了针对骨骼肌特异性SERCA1蛋白的单克隆抗体制备，验证了抗体对快肌纤维的特异性识别，并证实其在肌肉分型和钙离子调控研究中的应用潜力。
2. **文献名称**：*"Altered expression of SERCA1 in muscular dystrophy: a comparative study using immunofluorescence and western blot analysis"*
**作者**：Rosenberg AS, et al.
**摘要**：通过SERCA1抗体进行免疫荧光和Western blot分析，揭示了肌营养不良症模型中SERCA1蛋白表达水平下降，提示其与疾病相关的钙稳态失调机制。
3. **文献名称**：*"Characterization of SERCA1 isoform-specific functions in skeletal muscle regeneration"*
**作者**：Choi KM, et al.
**摘要**：利用SERCA1特异性抗体研究骨骼肌再生过程，发现SERCA1在肌细胞分化中的动态表达变化，并证实其在维持肌细胞钙平衡中的关键作用。
4. **文献名称**：*"Validation of commercial SERCA1 antibodies for use in human and animal tissue studies"*
**作者**：Garcia-Carreno LC, et al.
**摘要**：系统评估了多种市售SERCA1抗体的特异性、灵敏度和交叉反应性，为不同物种(如小鼠、人类)组织研究提供了抗体选择依据。
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如需获取具体文献，建议通过 **PubMed** 或 **Google Scholar** 搜索关键词(如"SERCA1 antibody validation"、"SERCA1 ATP2A1 immunohistochemistry")，并筛选涉及该抗体应用的实验研究。部分抗体厂商(如Abcam、Cell Signaling Technology)的产品说明书也会列出相关参考文献。
The sarco/endoplasmic reticulum Ca²⁺-ATPase 1 (SERCA1) is a P-type ATPase pump critical for calcium homeostasis in skeletal muscle. Encoded by the ATP2A1 gene, SERCA1 is predominantly expressed in fast-twitch skeletal muscle fibers, where it transports cytosolic Ca²⁺ back into the sarcoplasmic reticulum (SR) during muscle relaxation, enabling repeated contraction-relaxation cycles. This 100–110 kDa transmembrane protein couples ATP hydrolysis to Ca²⁺ translocation, maintaining low cytosolic Ca²⁺ levels essential for proper muscle function.
SERCA1-specific antibodies are vital tools for studying skeletal muscle physiology and pathology. They enable the detection, localization, and quantification of SERCA1 in tissues or cell lysates via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Researchers use these antibodies to investigate SERCA1 expression changes in muscular disorders, such as Brody myopathy (linked to ATP2A1 mutations), muscular dystrophy, or age-related sarcopenia. Additionally, they help characterize animal models of calcium dysregulation or evaluate therapeutic interventions targeting Ca²⁺ handling.
Commercial SERCA1 antibodies are typically raised against epitopes in conserved regions (e.g., cytoplasmic ATP-binding domains) and validated for specificity across species (human, mouse, rat). Some distinguish SERCA1 from isoforms like SERCA2 (cardiac/smooth muscle) or SERCA3 (non-muscle tissues). Proper controls, including tissue-specific lysates or knockout validation, are recommended to confirm antibody specificity in experimental settings.
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