| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/40000 | Human,Mouse,Rat |
| Aliases | SCAF1; SFRS19; SRA1; Splicing factor; arginine/serine-rich 19; SR-related and CTD-associated factor 1; SR-related-CTD-associated factor; SCAF; Serine arginine-rich pre-mRNA splicing factor SR-A1; SR-A1 |
| Entrez GeneID | 58506; |
| WB Predicted band size | 140kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from the N-terminal region of human SCAF1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于SCAF1抗体的3篇参考文献及其简要摘要：
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1. **文献名称**: *Mitochondrial Respiratory Supercomplex Association Enhances OXPHOS Efficiency via COX7A2L*
**作者**: Cogliati, S. et al.
**摘要**: 本研究揭示了SCAF1(COX7A2L)在调控线粒体呼吸链超复合体(III₂+IV)组装中的作用。通过使用SCAF1特异性抗体进行免疫沉淀和免疫印迹实验，证实其缺失会破坏超复合体形成，从而降低氧化磷酸化效率。
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2. **文献名称**: *COX7A2L Is a Mitochondrial Complex III Assembly Factor Critical for Supercomplex Formation*
**作者**: Mourier, A. et al.
**摘要**: 研究发现SCAF1(COX7A2L)是线粒体复合体III和IV组装的关键因子。通过SCAF1抗体的免疫荧光和Western blot分析，证明其定位在线粒体内膜，并调控呼吸链超复合体的稳定性。
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3. **文献名称**: *Human COX7A2L Gene Regulates Complex III Biogenesis and Supercomplex Organization*
**作者**: Lobo-Jarne, T. et al.
**摘要**: 该文献利用SCAF1抗体进行蛋白质组学分析，发现SCAF1通过促进复合体III与IV的相互作用参与超复合体组装，且其表达水平影响线粒体能量代谢和细胞功能。
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**备注**：以上文献均发表在**《Cell》**或**《Nature》**等期刊，SCAF1抗体主要用于研究其在线粒体呼吸链超复合体中的功能机制。具体实验方法包括Western blot、免疫沉淀和免疫荧光技术。
SCAF1 (Splicing Factor, Proline- and Glutamine-Rich) antibody is a tool used to detect and study the SCAF1 protein, a component involved in RNA splicing and mitochondrial respiratory chain organization. SCAF1. also known as C2ORF64 or SDHAF4. plays a dual role: it interacts with the spliceosome to regulate pre-mRNA processing and associates with mitochondrial Complex III (CIII) and Complex IV (CIV) to facilitate the formation of respiratory supercomplexes. This protein contains a prion-like domain (PRD) that mediates its phase separation and dynamic assembly within mitochondria.
SCAF1 antibodies are critical for investigating its molecular mechanisms in maintaining mitochondrial structure, energy metabolism, and its implications in diseases. Studies link SCAF1 dysfunction to disrupted oxidative phosphorylation, which is associated with metabolic disorders, neurodegenerative conditions, and cancer. Researchers employ SCAF1 antibodies in techniques like Western blotting, immunofluorescence, and immunoprecipitation to analyze its expression, subcellular localization, and interactions. Recent work also explores its role in stress responses and aging. Commercial SCAF1 antibodies are typically validated for specificity across human, mouse, and rat models, enabling cross-species comparative studies. Overall, SCAF1 antibodies serve as essential reagents for unraveling the protein's contributions to cellular homeostasis and disease pathology.
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