| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/40000 | Human,Mouse,Rat |
| Aliases | RPL27A; 60S ribosomal protein L27a |
| Entrez GeneID | 6157; |
| WB Predicted band size | 20kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from the C-terminal region of human Ribosomal Protein L27A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于 Ribosomal Protein L27A 抗体的参考文献示例(注：以下内容为示例性概括，非真实文献)：
1. **"Role of Ribosomal Protein L27A in Tumor Progression and Its Antibody-Based Detection"**
- **作者**: Smith J, et al.
- **摘要**: 研究利用 RPL27A 特异性抗体分析其在多种癌症组织中的表达水平，发现 RPL27A 在乳腺癌中显著高表达，并与肿瘤侵袭性相关。
2. **"Structural Characterization of Ribosomal Protein L27A Using Monoclonal Antibodies"**
- **作者**: Zhang Y, et al.
- **摘要**: 通过开发针对 RPL27A 的单克隆抗体，探究其在核糖体大亚基组装中的功能，揭示其与 rRNA 结合的分子机制。
3. **"RPL27A Antibody as a Tool for Studying Ribosome Biogenesis in Yeast"**
- **作者**: Tanaka M, et al.
- **摘要**: 利用 RPL27A 抗体进行免疫沉淀实验，验证其在酵母核糖体合成中的关键作用，并发现其缺失导致翻译效率下降。
4. **"Cross-Species Reactivity of Anti-RPL27A Antibody in Developmental Biology Studies"**
- **作者**: Lee H, et al.
- **摘要**: 评估 RPL27A 抗体在斑马鱼和小鼠模型中的交叉反应性，证明其在胚胎发育过程中对蛋白质合成的调控功能。
如需真实文献，建议通过 PubMed 或 Google Scholar 检索关键词“RPL27A antibody”或结合具体研究场景(如疾病模型、结构生物学等)。
The ribosomal protein L27A (RPL27A) is a component of the 60S large ribosomal subunit, playing a critical role in protein synthesis by stabilizing rRNA structure and facilitating peptidyl transferase activity during translation. As part of the ribosome, it contributes to the assembly and function of the ribosomal machinery, ensuring accurate polypeptide chain elongation. RPL27A is ubiquitously expressed across tissues, reflecting its essential role in cellular homeostasis.
Antibodies targeting RPL27A are widely used in research to study ribosome biogenesis, translation regulation, and cellular stress responses. These antibodies enable the detection and quantification of RPL27A in techniques like Western blotting (WB), immunofluorescence (IF), and immunohistochemistry (IHC). They are particularly valuable in investigating diseases linked to ribosomal dysfunction, such as cancer, developmental disorders, or ribosomopathies, where altered RPL27A expression may serve as a biomarker.
Commercial RPL27A antibodies are typically validated for specificity using knockout cell lines or siRNA-mediated knockdown to confirm target absence. Applications often include studying ribosome assembly defects, translational control under stress (e.g., nutrient deprivation), or crosstalk between ribosomal proteins and signaling pathways like mTOR. Researchers should optimize antibody dilutions and validate protocols for each experimental system, as ribosomal proteins can exhibit high conservation across species, affecting cross-reactivity.
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