| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | CDCA2; Cell division cycle-associated protein 2; Recruits PP1 onto mitotic chromatin at anaphase protein; Repo-Man |
| Entrez GeneID | 157313; |
| WB Predicted band size | 113kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from the Internal region of human Repo-Man. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Repo-Man抗体的3篇参考文献，包括文献名称、作者及简要摘要：
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1. **文献名称**: *Repo-Man coordinates chromosomal reorganization with nuclear envelope reassembly during mitotic exit*
**作者**: Vagnarelli, P., Ribeiro, S., Sennels, L., et al.
**摘要**: 研究利用Repo-Man抗体进行免疫荧光定位，发现Repo-Man在有丝分裂退出时协调染色体重组与核膜重新组装，并揭示其通过招募蛋白磷酸酶PP1γ调控核膜蛋白的去磷酸化。
2. **文献名称**: *Repo-Man recruits PP1γ to chromatin and is essential for nuclear envelope assembly*
**作者**: Trinkle-Mulcahy, L., Andersen, J., Lam, Y.W., et al.
**摘要**: 通过Repo-Man抗体的免疫沉淀实验，证明Repo-Man在核膜组装过程中招募PP1γ至染色质，并发现其缺失导致核膜结构异常，提示其在细胞分裂中的关键作用。
3. **文献名称**: *Repo-Man interacts with Ki-67 and regulates mitotic chromosome architecture*
**作者**: Booth, D.G., Takagi, M., Sanchez-Pulido, L., et al.
**摘要**: 结合Repo-Man抗体和Ki-67染色，研究发现Repo-Man与Ki-47相互作用，共同维持有丝分裂染色体的空间结构，并影响染色体分离的保真度。
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以上研究均通过Repo-Man抗体揭示其在细胞周期调控、核膜组装及染色体动力学中的功能。如需具体期刊或年份，建议通过PubMed或学术数据库进一步检索。
Repo-Man antibodies are essential tools in studying the dynamic processes of mitosis and nuclear organization. Repo-Man (Recruitment Protein of PP1 in Mitosis), a scaffold protein, plays a critical role in coordinating mitotic exit by recruiting protein phosphatase 1 (PP1) to chromatin. This interaction facilitates dephosphorylation of key mitotic substrates, enabling chromatin decondensation and nuclear envelope reformation. Discovered in the mid-2000s, Repo-Man is also implicated in interphase nuclear functions, including DNA damage response and chromosomal stability. Its dysregulation has been linked to cancers, where aberrant expression correlates with genomic instability.
Repo-Man antibodies, typically raised in rabbits or mice, enable researchers to investigate the protein’s localization, expression, and interactions via techniques like immunofluorescence, Western blotting, and immunoprecipitation. These antibodies have been pivotal in uncovering Repo-Man’s dual roles: during mitosis, it anchors PP1 to regulate chromatin remodeling, while in interphase, it associates with nuclear pore components and DNA repair machinery. Studies using Repo-Man antibodies have also highlighted its phosphorylation-dependent regulation and crosstalk with Aurora kinases. By elucidating Repo-Man’s mechanisms, these antibodies contribute to understanding cell cycle control, nuclear architecture, and potential therapeutic targets for diseases linked to mitotic errors.
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