Cat: PA1000-2204

Recombinant Human NT4 Protein,His

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关键信息

  • 基因名

    NT4

  • 应用

    SPRMSTBLIITCELISA细胞实验药物筛选

  • 别名

    NTF4;NTF5;Neurotrophin-4

  • 种属

    Human

  • 表达系统

    E. coli

  • 标签

    His tag N-Terminus

  • 纯度

    Greater than 90% as determined by SDS-PAGE.

  • 蛋白编号

    P34130

  • 表达区间

    81-210aa

  • 氨基酸序列

    M+GVSETAPASRRGELAVCDAVSGWVTDRRTAVDLRGREVEVLGEVPAAG GSPLRQYFFETRCKADNAEEGGPGAGGGGCRGVDRRHWVSECKAKQSYVR ALTADAQGRVGWRWIRIDTACVCTLLSRTGRA

  • 分子量

    28 kDa

  • 内毒素

    < 1.0 EU per μg protein as determined by the LAL method.

  • 性状

    Freeze-dried powder

  • 缓冲液

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • 复溶方法

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • 个性化定制

    点位突变 标签定制 buffer定制 全长蛋白定制

  • 稳定性测试

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • 保存条件 & 期限

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • 运输条件

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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背景信息

NT4, also known as Neurotrophin-4, is a member of the neurotrophin family, which plays a crucial role in the growth, survival, and differentiation of neurons in the nervous system. The research on NT4 and its recombinant protein forms has gained significant attention due to its potential therapeutic applications in neurodegenerative diseases, nerve injuries, and various neurological disorders. NT4 functions through its receptor, TrkB (tropomyosin receptor kinase B), which is involved in signaling pathways that support neuronal health and function. Unlike other neurotrophins, NT4 is particularly beneficial for certain types of neurons, promoting their survival and enhancing synaptic plasticity. The production of recombinant NT4 protein allows for detailed studies of its effects on neuronal growth and regeneration, paving the way for potential treatment options. Advances in genetic engineering techniques, such as recombinant DNA technology, enable the efficient production and purification of NT4, facilitating various in vitro and in vivo studies. These investigations aim to elucidate NT4's mechanisms of action, optimize its pharmacological properties, and assess its effectiveness in preclinical models. Insights gained from this research not only deepen our understanding of neurotrophic factors but also contribute to the development of innovative therapies to combat debilitating neurological conditions. Overall, the study of NT4 recombinant proteins holds promise for the advancement of neurobiology and neurotherapeutics, addressing a critical need for effective treatments in neurology.

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