| WB | 咨询技术 | Human,Monkey |
| IF | 咨询技术 | Human,Monkey |
| IHC | 咨询技术 | Human,Monkey |
| ICC | 技术咨询 | Human,Monkey |
| FCM | 咨询技术 | Human,Monkey |
| Elisa | 1/10000 | Human,Monkey |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human GLP1R |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于Olfactory receptor 4K14(OR4K14)抗体的假设性参考文献示例(注：实际文献可能需要通过学术数据库验证)：
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1. **"A novel antibody for olfactory receptor OR4K14 reveals its expression in human olfactory epithelium"**
- **作者**: Smith J, et al.
- **摘要**: 本研究开发了一种针对OR4K14的高特异性多克隆抗体，通过免疫组化和Western blot验证其在人类嗅觉上皮中的表达，证实其在嗅觉信号传导中的潜在作用。
2. **"OR4K14 antibody-based detection of ectopic expression in non-olfactory tissues"**
- **作者**: Zhang L, et al.
- **摘要**: 利用OR4K14抗体探索该受体在小鼠睾丸和肺组织中的非嗅觉表达，提示其可能在生殖或免疫调节中具有未知功能。
3. **"Antibody validation for olfactory receptors: A case study of OR4K14 in neurodegenerative disease"**
- **作者**: García R, et al.
- **摘要**: 通过免疫荧光和流式细胞术验证OR4K14抗体特异性，并发现阿尔茨海默病患者脑脊液中该受体的表达异常，可能与疾病进程相关。
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**建议**：若需真实文献，可通过PubMed或Google Scholar搜索关键词“OR4K14 antibody”或“olfactory receptor 4K14”，筛选涉及抗体开发、验证或应用的实验研究。部分研究可能未在摘要中明确提及抗体，需结合全文方法部分确认。
The olfactory receptor 4K14 (OR4K14) antibody is a tool used to study the OR4K14 protein, a member of the olfactory receptor (OR) family. ORs are G protein-coupled receptors (GPCRs) primarily expressed in olfactory sensory neurons, where they detect odorant molecules and initiate signal transduction. OR4K14. encoded by the OR4K14 gene in humans, is part of a large, evolutionarily conserved gene family with over 400 functional receptors in humans. These receptors are characterized by seven transmembrane domains and exhibit high sequence diversity, enabling the detection of a wide range of odorants.
The OR4K14 antibody is typically developed using recombinant protein fragments or synthetic peptides corresponding to specific regions of the OR4K14 receptor. It is widely employed in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect OR4K14 expression in tissues or cell lines. While ORs are predominantly studied in the olfactory system, emerging research suggests their ectopic expression in non-olfactory tissues (e.g., brain, heart, or immune cells), implicating roles in cellular processes like proliferation, migration, or metabolic regulation. The OR4K14 antibody thus aids in exploring these non-canonical functions.
Challenges in developing OR antibodies include high sequence homology among OR subtypes and low endogenous protein levels. Validation via knockout controls or orthogonal methods is critical to confirm specificity. Research using this antibody contributes to understanding olfactory signaling mechanisms, tissue-specific OR roles, and potential therapeutic targets in diseases linked to OR dysregulation.
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