关键信息
-
基因名
LTB4DH
- 应用
-
别名
15-oxoprostaglandin 13-reductase; FLJ99229; leukotriene B4 12-hydroxydehydrogenase; LTB4DH; MGC34943; NADP-dependent leukotriene B4 12-hydroxydehydrogenase; PGR1; PRG-1; Prostaglandin reductase 1; Ptgr1; PTGR1_HUMAN; RP11-16L21.1; ZADH3; zinc binding alcohol dehydrogenase domain containing 3
-
种属
Human
-
表达系统
E. coli
-
标签
GST-tag at N-terminal
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q14914
-
表达区间
1-329aa
-
氨基酸序列
MVRTKTWTLK KHFVGYPTNS DFELKTAELP PLKNGEVLLE ALFLTVDPYM RVAAKRLKEG DTMMGQQVAK VVESKNVALP KGTIVLASPG WTTHSISDGK DLEKLLTEWP DTIPLSLALG TVGMPGLTAY FGLLEICGVK GGETVMVNAA AGAVGSVVGQ IAKLKGCKVV GAVGSDEKVA YLQKLGFDVV FNYKTVESLE ETLKKASPDG YDCYFDNVGG EFSNTVIGQM KKFGRIAICG AISTYNRTGP LPPGPPPEIV IYQELRMEAF VVYRWQGDAR QKALKDLLKW VLEGKIQYKE YIIEGFENMP AAFMGMLKGD NLGKTIVKA
-
分子量
35.8 KDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
LTB4DH (leukotriene B4 dehydrogenase) is an important enzyme involved in the metabolism of leukotrienes, which are lipid mediators playing crucial roles in inflammatory responses and immune regulation. Dysregulation of leukotriene signaling has been implicated in various pathological conditions, including asthma, cardiovascular diseases, and autoimmune disorders. Research into LTB4DH has garnered significant attention due to its potential impact on modulating inflammation. Specifically, LTB4 is a pro-inflammatory leukotriene that can exacerbate tissue damage and promote chronic inflammation. LTB4DH catalyzes the conversion of LTB4 to a less active metabolite, thereby serving as a critical regulatory point in leukotriene biosynthesis. Understanding the structure and function of LTB4DH through recombinant protein studies can shed light on its enzymatic mechanisms and regulatory pathways. Furthermore, recombinant LTB4DH can be used in drug development and therapeutic applications aimed at controlling excessive inflammation. Current studies also focus on the protein's potential as a biomarker for inflammatory diseases and its role in shaping immune responses. Overall, the exploration of LTB4DH as a recombinant protein offers promising avenues for enhancing our understanding of inflammation and developing novel therapeutic strategies for managing related diseases.
