| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/40000 | Human,Mouse,Rat |
| Aliases | NEIL3; Endonuclease 8-like 3; DNA glycosylase FPG2; DNA glycosylase/AP lyase Neil3; Endonuclease VIII-like 3; Nei-like protein 3 |
| Entrez GeneID | 55247; |
| WB Predicted band size | 35kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from the C-terminal region of human NEIL3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于NEIL3抗体的3篇参考文献的简要概括：
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1. **文献名称**：NEIL3 promotes glioblastoma tumorigenesis by stabilizing the ALKBH2 demethylase
**作者**：Liu M. et al.
**摘要**：该研究揭示了NEIL3通过稳定去甲基化酶ALKBH2促进胶质母细胞瘤的恶性进展。研究利用NEIL3抗体进行免疫共沉淀实验，发现NEIL3与ALKBH2相互作用并抑制其泛素化降解，从而增强肿瘤细胞的DNA损伤修复能力。
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2. **文献名称**：NEIL3-dependent base excision repair regulates cellular replication stress in cancer stem cells
**作者**：Tahbaz N. et al.
**摘要**：本文通过NEIL3抗体介导的Western blot和免疫荧光技术，证明NEIL3在癌症干细胞中高表达，并通过修复复制应激导致的DNA损伤维持基因组稳定性。敲低NEIL3可显著抑制肿瘤干细胞的自我更新能力。
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3. **文献名称**：Structural and functional characterization of human NEIL3 in oxidative DNA damage repair
**作者**：Sejersted Y. et al.
**摘要**：研究解析了NEIL3蛋白的晶体结构，并利用NEIL3抗体验证其在细胞内的定位及功能。实验表明NEIL3特异性识别并修复氧化性DNA损伤(如8-oxoG)，为神经退行性疾病中的DNA修复缺陷提供了机制解释。
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以上研究均通过NEIL3抗体开展功能验证，涵盖肿瘤发生、干细胞维持及DNA修复机制等领域。如需具体文献年份或期刊，可进一步补充检索。
NEIL3 (Endonuclease VIII-like 3) is a DNA glycosylase belonging to the NEIL family, which plays a critical role in repairing oxidative DNA damage through the base excision repair (BER) pathway. Unlike other NEIL members (NEIL1 and NEIL2), NEIL3 exhibits unique substrate specificity, preferentially targeting and excising oxidized bases in single-stranded or bubble DNA structures, making it essential for resolving replication-associated DNA damage. It is particularly active during the S phase of the cell cycle and in proliferating cells, including cancer and stem cells. NEIL3 also participates in maintaining genomic stability, telomere integrity, and immune regulation, with implications in cancer, neurodegeneration, and aging.
NEIL3 antibodies are crucial tools for studying its expression, localization, and function. They enable detection via techniques like Western blot, immunofluorescence, and immunohistochemistry, aiding research into NEIL3's overexpression in cancers (e.g., glioblastoma, leukemia) and its role in neurodevelopment. These antibodies help elucidate molecular mechanisms linking NEIL3 dysfunction to disease, offering potential for therapeutic targeting. Validation for specificity and application in diverse experimental models remains a focus to ensure reliability in both basic and translational studies.
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