Rabbit Polyclonal NEDL2 Antibody

The NEDD4L2 antibody is a crucial tool for studying NEDD4L (Neural Precursor Cell Expressed Developmentally Downregulated 4-Like 2), an E3 ubiquitin ligase within the NEDD4 family. NEDD4L2 regulates protein ubiquitination, a process critical for protein degradation, cellular trafficking, and signaling. It contains a C-terminal HECT domain (responsible for ubiquitin transfer) and N-terminal WW domains (mediating substrate interactions). Dysregulation of NEDD4L2 is linked to hypertension, cancer progression, and neurological disorders due to its role in modulating ion channels (e.g., ENaC), growth factor receptors (e.g., EGFR), and synaptic proteins. The antibody enables detection and functional analysis of NEDD4L2 in diverse experimental settings, including Western blotting, immunofluorescence, and immunohistochemistry. Researchers use it to explore NEDD4L2’s phosphorylation-dependent activity (e.g., SGK1-mediated regulation) and its interactions with substrates like SMADs or PTEN. Both monoclonal and polyclonal variants exist, often validated using knockout controls to ensure specificity. Its applications extend to studying diseases such as epithelial cancers, where NEDD4L2 may act as a tumor suppressor, or neurodegenerative conditions involving aberrant protein turnover. Proper antibody validation remains essential due to potential cross-reactivity with homologous NEDD4 family members.