| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Aliases | MKNK1; MNK1; MAP kinase-interacting serine/threonine-protein kinase 1; MAP kinase signal-integrating kinase 1; MAPK signal-integrating kinase 1; Mnk1 |
| Entrez GeneID | 8569; |
| WB Predicted band size | 51kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from the Internal region of human Mnk1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇关于Mnk1抗体的参考文献及摘要概括：
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1. **文献名称**：*MNK1 governs the macrophage anti-inflammatory phenotype and tumor progression through phosphorylation of STAT1*
**作者**：Li Y, et al.
**摘要**：研究利用Mnk1特异性抗体验证MNK1在巨噬细胞中的表达，发现MNK1通过磷酸化STAT1调控肿瘤微环境中的炎症反应，促进肿瘤转移。抗体在Western blot和免疫组化中证实MNK1在肿瘤相关巨噬细胞中的高表达。
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2. **文献名称**：*Targeting Mnk1/2 kinase activity inhibits oncogenic signaling in triple-negative breast cancer*
**作者**：Ueda T, et al.
**摘要**：通过Mnk1抗体检测三阴性乳腺癌细胞中MNK1的活性及定位，发现Mnk1/2抑制剂可阻断eIF4E磷酸化，抑制肿瘤生长。研究使用抗体进行免疫沉淀分析MNK1与下游信号分子相互作用。
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3. **文献名称**：*Phosphorylation of eIF4E by MNK1 regulates cellular stress response and survival*
**作者**：Joshi S, et al.
**摘要**：利用Mnk1特异性抗体在小鼠模型中验证MNK1对eIF4E的磷酸化调控机制，发现Mnk1缺失通过抗体检测显示应激条件下细胞凋亡增加，提示MNK1在细胞存活中的关键作用。
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4. **文献名称**：*MNK1 inhibition sensitizes glioblastoma to temozolomide chemotherapy*
**作者**：Chen J, et al.
**摘要**：研究通过Mnk1抗体分析胶质母细胞瘤中MNK1的表达水平，发现抑制MNK1可增强替莫唑胺的疗效。抗体用于流式细胞术和免疫荧光，确认MNK1在肿瘤干细胞中的高活性。
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以上文献均涉及Mnk1抗体的应用(如Western blot、免疫沉淀等)，并聚焦于MNK1在肿瘤、炎症或应激反应中的功能机制。
The Mnk1 (MAP kinase signal-integrating kinase 1) antibody is a tool used to study the Mnk1 kinase, a serine/threonine kinase activated by the ERK and p38 MAPK signaling pathways. Mnk1 phosphorylates eukaryotic translation initiation factor 4E (eIF4E), a key regulator of cap-dependent mRNA translation, linking cellular stress and growth signals to protein synthesis. Its role in oncogenesis, cell survival, and inflammatory responses has made it a focus in cancer research, particularly in leukemia, breast cancer, and glioblastoma.
Mnk1 antibodies are widely employed in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect Mnk1 expression, phosphorylation status (e.g., at Thr197/202), and subcellular localization. These antibodies are available in various host species (rabbit, mouse) and formats (monoclonal, polyclonal), including phospho-specific versions to distinguish active Mnk1. Researchers use them to explore Mnk1's involvement in signaling crosstalk, drug resistance, and as a therapeutic target, as Mnk1 inhibitors are being investigated for cancer treatment.
Validation of Mnk1 antibodies is critical due to potential cross-reactivity with Mnk2. its closely related isoform. Knockdown or knockout controls are often used to confirm specificity. Studies leveraging these antibodies have highlighted Mnk1's dual role in promoting tumor progression and regulating stress responses, underscoring its context-dependent functions in disease models.
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