| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Aliases | MAP3K3; MAPKKK3; MEKK3; Mitogen-activated protein kinase kinase kinase 3; MAPK/ERK kinase kinase 3; MEK kinase 3; MEKK 3 |
| Entrez GeneID | 4215; |
| WB Predicted band size | 71kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from the Internal region of human MEK Kinase-3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于MEK Kinase-3(MEK3/MAP2K3)抗体的3篇文献示例(内容基于学术研究的典型方向概括，非真实文献)：
1. **文献名称**：*Development and Validation of a MEK3-Specific Monoclonal Antibody for Western Blot Analysis*
**作者**：Smith J, et al.
**摘要**：该研究报道了一种针对MEK3激酶的高特异性单克隆抗体的开发，通过免疫印迹验证其在多种细胞系中的敏感性及特异性，并确认其可用于检测磷酸化和非磷酸化形式的MEK3.
2. **文献名称**：*MEK3 Antibody-Based Immunoprecipitation Reveals Its Role in p38 MAPK Pathway Activation*
**作者**：Lee H, et al.
**摘要**：利用MEK3抗体进行免疫共沉淀实验，揭示了MEK3在炎症反应中通过激活p38 MAPK信号通路的作用机制，并证明其在氧化应激条件下的表达变化。
3. **文献名称**：*A Novel MEK3 Antibody for Immunohistochemical Detection in Human Tumor Tissues*
**作者**：Garcia R, et al.
**摘要**：研究团队开发了一种适用于石蜡包埋组织切片的MEK3抗体，验证其在多种癌症组织中的表达模式，并发现MEK3高表达与乳腺癌患者预后不良相关。
4. **文献名称**：*Functional Characterization of MEK3 Knockout Mice Using Phospho-Specific Antibodies*
**作者**：Wang Y, et al.
**摘要**：通过磷酸化MEK3抗体分析基因敲除小鼠模型，证实MEK3在胚胎发育和心脏功能中的关键作用，并阐明其与ERK1/2信号通路的交互关系。
(注：以上内容为模拟学术文献的典型描述，实际文献需通过PubMed、Web of Science等平台查询。)
MEK Kinase-3 (MEKK3), also known as MAP3K3. is a serine/threonine protein kinase belonging to the mitogen-activated protein kinase kinase kinase (MAP3K) family. It plays a critical role in the MAPK signaling cascade, particularly in the activation of the MEK5-ERK5 pathway. MEKK3 is involved in regulating diverse cellular processes, including proliferation, differentiation, apoptosis, and stress responses. It interacts with upstream regulators like G-proteins and adaptor proteins, and its activation is triggered by various stimuli, such as cytokines, growth factors, and cellular stress. Dysregulation of MEKK3 has been implicated in pathological conditions, including inflammation, cardiovascular diseases, and cancer, where it may promote tumor progression or angiogenesis.
Antibodies targeting MEKK3 are essential tools for studying its expression, localization, and function in both physiological and disease contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, immunoprecipitation, and immunofluorescence to detect MEKK3 in cell lines, tissues, or *in vivo* models. High-quality MEKK3 antibodies exhibit specificity for distinct phosphorylation states or conformational epitopes, enabling researchers to investigate activation-dependent signaling dynamics. Validation via knockout controls or siRNA-mediated silencing is critical to confirm antibody specificity. Commercial MEKK3 antibodies are often developed in hosts like rabbits or mice, with formats including monoclonal or polyclonal options. Their applications extend to translational research, such as identifying MEKK3 as a biomarker or therapeutic target in cancers characterized by aberrant MAPK pathway activity.
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