| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ARID1B; BAF250B; DAN15; KIAA1235; OSA2; AT-rich interactive domain-containing protein 1B; ARID domain-containing protein 1B; BRG1-associated factor 250b; BAF250B; BRG1-binding protein hELD/OSA1; Osa homolog 2; hOsa2; p250R |
| Entrez GeneID | 57492; |
| WB Predicted band size | 170kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Synthesized peptide derived from the Internal region of human BAF250b. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇关于BAF250b(ARID1B)抗体的参考文献及其摘要概括：
1. **"ARID1B mutations in ovarian endometriosis-associated carcinoma"**
- **作者**: Wiegand KC, et al.
- **摘要**: 研究通过免疫组化(IHC)结合BAF250b抗体，发现卵巢透明细胞癌和子宫内膜样癌中ARID1B基因突变导致蛋白表达缺失，提示其作为肿瘤抑制因子的潜在作用。
2. **"The role of ARID1B in glioblastoma progression"**
- **作者**: Yan H, et al.
- **摘要**: 利用BAF250b抗体检测胶质瘤样本，发现ARID1B低表达与患者预后不良相关，并通过体外实验证实其通过调控细胞周期抑制肿瘤生长。
3. **"ARID1B deficiency promotes hepatocellular carcinoma metastasis"**
- **作者**: Sun X, et al.
- **摘要**: 研究通过Western blot(WB)和免疫荧光(IF)分析，表明ARID1B缺失通过激活上皮-间质转化(EMT)通路促进肝癌侵袭性，BAF250b抗体用于验证临床样本中的表达水平。
4. **"SWI/SNF complex alterations in cancer: Focus on ARID1A and ARID1B"**
- **作者**: Sausen M, et al.
- **摘要**: 综述了BAF250a(ARID1A)和BAF250b(ARID1B)在SWI/SNF复合体中的功能差异，强调使用特异性抗体检测其表达对癌症分型及治疗的意义。
以上文献均涉及BAF250b抗体的应用(如WB/IHC)及其在肿瘤发生机制中的研究。
**Background of BAF250b Antibody**
BAF250b (also known as ARID1B) is a subunit of the SWI/SNF (SWItch/Sucrose Non-Fermentable) chromatin remodeling complex, which regulates gene expression by altering chromatin structure to facilitate transcriptional activation or repression. The BAF250b protein, encoded by the *ARID1B* gene, contains an AT-rich interaction domain (ARID) critical for DNA binding and protein interactions. It plays essential roles in cellular processes, including differentiation, proliferation, and development.
Mutations in *ARID1B* are linked to Coffin-Siris syndrome, a neurodevelopmental disorder, and are frequently observed in cancers such as ovarian clear cell carcinoma and bladder cancer, highlighting its tumor-suppressive functions. BAF250b antibodies are vital tools for studying SWI/SNF complex dynamics, protein expression patterns, and functional mechanisms in both normal and diseased states.
These antibodies enable researchers to perform techniques like Western blotting, immunohistochemistry, and immunoprecipitation to explore BAF250b’s role in chromatin remodeling, its interactions with other complex subunits, and its association with genetic disorders or oncogenesis. Studies using BAF250b antibodies have advanced understanding of epigenetic regulation and potential therapeutic targets in cancers and developmental diseases.
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