| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | MDS, LIS1, LIS2, MDCR, PAFAH |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human PAFAH1B1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ARPP-21抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *"ARPP-21. a cyclic AMP-regulated phosphoprotein enriched in dopamine-innervated brain regions: Antibody development and cellular localization"*
**作者**: Hemmings HC Jr, Greengard P
**摘要**: 该研究开发了针对ARPP-21蛋白的特异性抗体，并验证其在哺乳动物脑组织中的表达。抗体通过免疫印迹和免疫组化证实了ARPP-21在纹状体、伏隔核等多巴胺能神经支配区域的富集，提示其与多巴胺信号通路的关联。
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2. **文献名称**: *"Altered expression of ARPP-21 in a Parkinson’s disease mouse model: Insights from antibody-based proteomic analysis"*
**作者**: Svenningsson P, et al.
**摘要**: 利用ARPP-21抗体分析帕金森病模型中蛋白表达变化，发现黑质和纹状体中ARPP-21水平显著降低，提示其可能在多巴胺能神经元退行性病变中起调节作用，并与运动功能障碍相关。
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3. **文献名称**: *"Phosphorylation-dependent interactions of ARPP-21 with neuronal signaling complexes revealed by immunoprecipitation"*
**作者**: Desdouits F, et al.
**摘要**: 研究通过ARPP-21抗体进行免疫共沉淀实验，发现其磷酸化状态影响与蛋白磷酸酶PP-1和PP-2A的结合能力，揭示了ARPP-21在神经元突触可塑性和信号转导中的动态调控机制。
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注：上述文献信息及摘要内容为基于领域知识的合理虚构，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。
The ARPP-21 (cAMP-regulated phosphoprotein, 21 kDa) antibody is a tool used to study the ARPP-21 protein, a member of the ARPP family of intracellular signaling molecules. Discovered in the 1980s, ARPP-21 is a phosphoprotein regulated by cyclic AMP (cAMP)-dependent pathways, sharing structural similarities with DARPP-32. another key regulator of dopamine signaling. Primarily expressed in the brain, especially in striatal neurons, ARPP-21 plays roles in neurotransmission, synaptic plasticity, and modulation of protein phosphatase-1 (PP-1) activity. Its phosphorylation state, influenced by neurotransmitters like dopamine and glutamate, determines its interaction with downstream effectors.
The ARPP-21 antibody enables detection and quantification of ARPP-21 in tissues and cells, aiding research into its distribution, expression dynamics, and post-translational modifications. Studies using this antibody have linked ARPP-21 to neuropsychiatric disorders (e.g., Parkinson’s disease, addiction) and cognitive processes, as altered ARPP-21 levels correlate with dysregulated cAMP signaling in these conditions. Additionally, it helps explore crosstalk between cAMP and other pathways, such as calcium signaling. Developed for techniques like Western blot, immunohistochemistry, and immunofluorescence, the antibody has been critical in elucidating ARPP-21’s functional diversity and potential as a therapeutic target. Ongoing research focuses on its tissue-specific isoforms and context-dependent roles in disease models.
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