| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/40000 | Human,Mouse,Rat |
| Aliases | SLC25A31; AAC4; ANT4; SFEC; ADP/ATP translocase 4; ADP; ATP carrier protein 4; Adenine nucleotide translocator 4; ANT 4; Solute carrier family 25 member 31; Sperm flagellar energy carrier protein |
| Entrez GeneID | 83447; |
| WB Predicted band size | 35kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from the Internal region of human ANT4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于ANT4抗体的3篇参考文献示例(内容基于学术知识库信息整理，非真实引用)：
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1. **文献名称**: "ANT4 Is Essential for Spermatogenesis in Mice"
**作者**: Mizuno, S. et al.
**摘要**: 研究证实ANT4在小鼠睾丸生殖细胞中特异性表达，通过基因敲除模型发现其缺失会导致精子发生停滞及雄性不育，表明ANT4是精子能量代谢的关键调控因子。
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2. **文献名称**: "Tissue-specific Expression of the Adenine Nucleotide Translocase Isoforms in Mammals"
**作者**: Hüttemann, M. et al.
**摘要**: 本文系统分析了ANT家族(ANT1-4)的组织分布，指出ANT4仅在睾丸和某些癌细胞中高表达，提示其可能作为生殖系统或癌症治疗的潜在靶点。
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3. **文献名称**: "Structural and Functional Characterization of the ANT4 Isoform in Mitochondrial Membrane Permeability"
**作者**: Kokoszka, J.E. et al.
**摘要**: 通过生化实验解析ANT4的跨膜结构域，发现其与其他ANT亚型相比具有独特的ATP/ADP转运特性，并参与线粒体膜通透性调节，可能与凋亡信号通路相关。
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**提示**：以上为模拟参考文献，实际研究需通过PubMed或Web of Science等平台检索真实文献(关键词：*ANT4 antibody, Adenine Nucleotide Translocase 4*)。
ANT4 (Adenine Nucleotide Translocase 4) is a mitochondrial inner membrane protein belonging to the solute carrier 25 (SLC25) family, which facilitates the exchange of ADP and ATP across the mitochondrial membrane, a critical process for cellular energy metabolism. Unlike other ANT isoforms (ANT1. ANT2. ANT3) that are widely expressed, ANT4 exhibits tissue-specific expression, primarily in the testes and during early embryonic development. This unique expression pattern suggests a specialized role in germ cell development and spermatogenesis. Studies indicate that ANT4 is essential for male fertility, as its knockout in mice leads to defective sperm maturation and infertility.
Structurally, ANT4 shares the conserved tripartite repeat structure of mitochondrial carriers but possesses distinct N-terminal domains that may regulate its function or localization. Beyond energy transport, ANT4 has been implicated in modulating mitochondrial apoptosis pathways by interacting with components of the mitochondrial permeability transition pore (mPTP). Dysregulation of ANT4 is associated with reproductive disorders and has been explored in cancer research due to its potential role in tumor cell survival.
As a research tool, ANT4-specific antibodies are vital for investigating its expression dynamics, subcellular localization, and mechanistic contributions to developmental and pathological processes. Their application spans immunohistochemistry, Western blotting, and functional studies in reproductive biology and mitochondrial medicine.
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