| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Aliases | ARRDC1; Arrestin domain-containing protein 1 |
| Entrez GeneID | 92714; |
| WB Predicted band size | 48kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from the Internal region of human Arrdc1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于ARRDC1抗体的示例参考文献(注：以下为模拟示例，实际文献需通过学术数据库检索确认)：
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1. **文献名称**：*ARRDC1 regulates tumor suppression through Notch1 ubiquitination*
**作者**：Smith J, et al.
**摘要**：研究揭示了ARRDC1通过泛素化调控Notch1信号通路抑制乳腺癌进展，利用ARRDC1抗体进行免疫共沉淀实验，证实其与Notch1的直接互作。
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2. **文献名称**：*Development and validation of a polyclonal antibody against human ARRDC1*
**作者**：Li Y, et al.
**摘要**：本文报道了一种针对人源ARRDC1蛋白的多克隆抗体的制备与验证，通过Western blot和免疫荧光证明其特异性，并应用于组织样本中ARRDC1表达水平的检测。
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3. **文献名称**：*ARRDC1 modulates cellular metabolism via AMPK signaling*
**作者**：Wang H, et al.
**摘要**：研究利用ARRDC1抗体进行免疫组化分析，发现ARRDC1缺失导致AMPK通路异常激活，进而影响肝脏脂质代谢，提示其在代谢疾病中的潜在作用。
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4. **文献名称**：*ARRDC1 mediates viral entry through clathrin-independent endocytosis*
**作者**：Garcia R, et al.
**摘要**：通过ARRDC1抗体敲低实验，证实ARRDC1在非网格蛋白依赖的内吞途径中关键作用，影响HIV等病毒的细胞入侵机制。
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建议通过PubMed或Google Scholar以“ARRDC1 antibody”或“ARRDC1 function”为关键词检索最新文献获取准确信息。
The ARRDC1 (Arrestin domain-containing protein 1) antibody is a tool used to detect and study the ARRDC1 protein, a member of the α-arrestin family. ARRDC1 plays a role in membrane trafficking, protein ubiquitination, and degradation, primarily via interactions with the endosomal sorting complex required for transport (ESCRT) pathway. It contains conserved arrestin-like domains, including PPxY and LPxY motifs, enabling binding to WW domain-containing proteins such as Nedd4-family E3 ubiquitin ligases. This interaction facilitates the ubiquitination of cargo proteins, targeting them for lysosomal degradation or extracellular vesicle secretion.
ARRDC1 is implicated in regulating cell surface receptors (e.g., EGFR), glucose metabolism, and cellular stress responses. Its dysfunction has been linked to cancer, metabolic disorders, and viral budding processes. The ARRDC1 antibody is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate its expression, localization, and mechanistic roles in these pathways.
Researchers often validate the antibody’s specificity using ARRDC1-knockout controls, as cross-reactivity with other arrestin family members can occur. Commercial antibodies are typically raised against epitopes within the N-terminal or C-terminal regions. Studies employing this antibody have advanced understanding of ARRDC1’s tumor-suppressive roles, involvement in exosome biogenesis, and regulation of autophagy, making it a critical reagent in cell biology and disease research.
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