Rabbit Polyclonal TF2H2 Antibody

The TFIIH (Transcription Factor II Human) complex is a critical multi-subunit protein assembly involved in transcription initiation and nucleotide excision repair (NER) in eukaryotic cells. Within this complex, the ERCC2/XPD and ERCC3/XPB subunits function as ATP-dependent DNA helicases, playing essential roles in unwinding DNA during transcription and damage recognition/repair. Antibodies targeting the ERCC2/XPD component (e.g., TF2H2 antibodies) are widely used tools to study TFIIH's dual roles in these processes. These antibodies are typically raised against conserved epitopes of human ERCC2/XPD, enabling applications such as Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate TFIIH localization, protein interactions, and regulatory mechanisms. Research utilizing TF2H2 antibodies has contributed to understanding transcription dysregulation and DNA repair deficiencies linked to diseases like xeroderma pigmentosum (XP) and trichothiodystrophy (TTD), which are associated with mutations in ERCC2/XPD. Additionally, these antibodies serve as biomarkers in studies exploring cancer therapies targeting DNA repair pathways, particularly in predicting responses to platinum-based chemotherapy. Validation of TF2H2 antibodies often involves siRNA knockdown or CRISPR-Cas9 knockout controls to confirm specificity for the ~87 kDa ERCC2/XPD protein.