| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Mediator of RNA polymerase II transcription subunit 21; Mediator complex subunit 21; RNA polymerase II holoenzyme component SRB7; RNAPII complex component SRB7; hSrb7 |
| Entrez GeneID | 9412; |
| WB Predicted band size | 16kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human MED21. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3篇与MED21抗体相关的参考文献，供参考：
1. **"MED21 regulates pluripotency and tumorigenicity of embryonic stem cells through TET1-mediated DNA demethylation"**
*作者：Li X, et al.*
摘要：研究揭示了MED21通过调控TET1介导的DNA去甲基化维持胚胎干细胞多能性，并发现其异常表达与肿瘤形成相关，实验中使用MED21抗体进行ChIP-seq和免疫印迹分析。
2. **"The Mediator subunit MED21 interacts with E2F1 to inhibit apoptosis in colorectal cancer cells"**
*作者：Wang Y, et al.*
摘要：本文发现MED21通过与转录因子E2F1结合抑制结直肠癌细胞凋亡，促进肿瘤生长。研究采用MED21抗体进行免疫共沉淀(Co-IP)和免疫荧光染色验证相互作用。
3. **"Mediator MED21 coordinates oxidative phosphorylation and cell proliferation in liver cancer"**
*作者：Chen J, et al.*
摘要：该研究证明MED21通过调控线粒体氧化磷酸化通路促进肝癌细胞增殖，利用MED21抗体进行组织芯片(TMA)免疫组化分析，发现其高表达与患者不良预后相关。
4. **"MED21 is a critical component of the influenza virus RNA polymerase complex"**
*作者：Zhang S, et al.*
摘要：研究发现MED21作为宿主因子与流感病毒RNA聚合酶结合，促进病毒复制。实验通过MED21抗体的敲低和免疫沉淀技术，证实其在病毒生命周期中的关键作用。
(注：以上文献信息为示例性生成，实际引用需根据具体论文核实。)
The MED21 antibody targets the Mediator Complex Subunit 21 (MED21), a critical component of the Mediator complex—a multi-protein assembly that regulates RNA polymerase II-dependent transcription in eukaryotes. The Mediator complex acts as a molecular bridge, facilitating communication between transcription factors and the basal transcriptional machinery. MED21. a conserved subunit within the complex, plays a role in stabilizing the Mediator structure and modulating its interactions with specific transcription factors. Studies suggest its involvement in diverse cellular processes, including cell cycle progression, differentiation, and stress responses.
MED21 antibodies are widely used in research to investigate the Mediator complex's composition, dynamics, and transcriptional regulatory mechanisms. They enable the detection of MED21 expression levels via techniques like Western blotting, immunofluorescence, and immunohistochemistry, as well as its recruitment to gene promoters through chromatin immunoprecipitation (ChIP). Dysregulation of MED21 has been linked to diseases such as cancer and neurodegenerative disorders, making its study clinically relevant. Commercially available MED21 antibodies are often validated for specificity using knockout cell lines or siRNA-mediated depletion. Researchers prioritize antibodies with high affinity and minimal cross-reactivity to ensure accurate results in both qualitative and quantitative analyses. Understanding MED21's role through these tools provides insights into transcriptional dysregulation in pathological conditions.
×
