| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Transducin-like enhancer protein 4; TLE4; KIAA1261; |
| Entrez GeneID | 7091; |
| WB Predicted band size | 83kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from internal of human TLE4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TLE4抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *TLE4 is a transcriptional corepressor in acute lymphoblastic leukemia*
**作者**: Wang, J., et al.
**摘要**: 本研究通过Western blot和免疫组化技术，利用TLE4抗体检测了其在急性淋巴细胞白血病(ALL)患者样本中的表达。研究发现TLE4通过抑制NOTCH信号通路相关基因的转录，发挥肿瘤抑制作用，并提示其可能作为ALL的潜在诊断标志物。
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2. **文献名称**: *Groucho/TLE family proteins function as transcriptional corepressors in multiple cancers*
**作者**: Chen, Y., & Zhang, L.
**摘要**: 该研究探讨了TLE家族蛋白(包括TLE4)在多种癌症中的调控机制。通过免疫沉淀和ChIP实验结合TLE4抗体，发现TLE4与HES1蛋白相互作用，共同抑制胶质母细胞瘤中促癌基因的表达，并证明其低表达与患者预后不良相关。
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3. **文献名称**: *Characterization of TLE4 antibody specificity in neural development studies*
**作者**: Smith, R., et al.
**摘要**: 文章评估了多种商业化TLE4抗体的特异性，通过敲除小鼠模型验证抗体可靠性。实验表明，特定TLE4抗体能有效标记发育中的小鼠大脑皮层神经元，为研究TLE4在神经分化中的转录抑制功能提供了工具支持。
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(注：上述文献为示例，实际引用需根据具体研究内容检索真实文献。)
The TLE4 antibody is a tool used to detect the Transducin-like enhancer of split 4 (TLE4) protein, a member of the TLE/Groucho family of transcriptional co-repressors. TLE proteins lack DNA-binding domains but interact with transcription factors, such as those in the TCF/LEF family, to regulate gene expression by recruiting histone deacetylases (HDACs) and chromatin-modifying complexes. TLE4 is particularly notable for its role in modulating Wnt/β-catenin signaling, where it suppresses target genes by forming complexes with DNA-bound repressors. This interaction is critical in developmental processes, including neurogenesis, hematopoiesis, and immune regulation, as well as in diseases like cancer, where dysregulated Wnt signaling drives tumor progression.
Antibodies against TLE4 are widely used in research to study its expression patterns, subcellular localization, and functional interactions. They are applied in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to explore TLE4's involvement in both normal physiology and pathology. For example, studies have linked altered TLE4 expression to leukemia, colorectal cancer, and neurological disorders, highlighting its potential as a biomarker or therapeutic target. However, challenges remain in ensuring antibody specificity due to high homology among TLE family members. Researchers often validate TLE4 antibodies using knockout controls or siRNA-based approaches to confirm target specificity in experimental models.
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