| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CF-1 50 kDa subunit; CSTF 50 kDa subunit; CSTF1; Cleavage stimulation factor; 50 kDa subunit |
| Entrez GeneID | 1477; |
| WB Predicted band size | 48kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from N-terminal of human CSTF1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及CSTF1抗体的相关文献概览：
1. **文献名称**: "CSTF1 regulates breast cancer metastasis and stemness through 3'UTR shortening of ZFP36L1"
**作者**: Li W, et al.
**摘要**: 本研究揭示CSTF1通过介导ZFP36L1基因的3'UTR缩短促进乳腺癌细胞转移和干性特征，实验中利用CSTF1抗体进行免疫印迹和免疫共沉淀验证其与mRNA加工复合物的相互作用。
2. **文献名称**: "Cleavage factor Im links transcription termination to DNA damage response"
**作者**: Hsu TY, et al.
**摘要**: 该研究发现CSTF1作为转录终止复合物成员，参与DNA损伤应答通路。研究使用CSTF1特异性抗体进行ChIP-seq分析，证明其在维持基因组稳定性中的功能。
3. **文献名称**: "Global profiling of 3'UTR cleavage and polyadenylation in human hematopoietic cells"
**作者**: Lin Y, et al.
**摘要**: 文章通过CSTF1抗体进行RNA免疫沉淀测序(RIP-seq)，系统性绘制了造血细胞中3'UTR加工位点图谱，揭示CSTF1在造血分化过程中调控mRNA多样性的机制。
备注：以上文献信息为模拟示例，实际引用时建议通过PubMed或Web of Science核对原文准确性。
The CSTF1 antibody targets the Cleavage Stimulation Factor 1 (CSTF1), a critical subunit of the Cleavage Stimulation Factor (CSTF) complex involved in mRNA 3'-end processing. The CSTF complex, composed of CSTF1. CSTF2. and CSTF3. facilitates transcription termination and polyadenylation by recognizing polyadenylation signals (PAS) in nascent RNA and recruiting poly(A) polymerases. CSTF1 stabilizes interactions between CSTF2 and RNA, ensuring precise cleavage and polyadenylation, which are essential for mRNA stability, nuclear export, and translation.
CSTF1 dysregulation is implicated in diseases, including cancers, where altered polyadenylation leads to oncogene overexpression or tumor suppressor silencing. It also interacts with viral proteins, aiding viral mRNA processing. CSTF1 antibodies are vital tools for studying mRNA maturation, gene expression mechanisms, and disease pathways. They are used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to assess CSTF1 expression, localization, and interactions. Research applications span cancer biology, virology, and neurobiology, highlighting its role in cellular homeostasis and pathology. Commercial CSTF1 antibodies are typically raised in rabbits or mice, validated for specificity via knockout controls.
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