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Rabbit Polyclonal IRS-1(Ab-312) Antibody

  • 中文名： IRS-1 (Ab-312)抗体
  • 别    名： Insulin receptor substrate-1; IRS-1; IRS1;
货号: IPDX41692
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 1/100-1/500 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesInsulin receptor substrate-1; IRS-1; IRS1;
Entrez GeneID3667;
WB Predicted band size180kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse
ImmunogenSynthesized non-phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 312 (A-T-S(p)-P-A).
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于IRS-1 (Ab-312)抗体的模拟参考文献示例(注：具体文献需通过学术数据库验证，以下为假设性示例)：

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1. **文献名称**: *"IRS-1 Tyrosine Phosphorylation in Insulin Signaling: Implications in Metabolic Disorders"*

**作者**: Smith, J. et al.

**摘要**: 本研究使用IRS-1 (Ab-312)抗体通过Western blot分析，探讨了胰岛素刺激下IRS-1酪氨酸磷酸化(如Tyr612位点)在肝脏和肌肉组织中的作用，揭示了其在2型糖尿病胰岛素抵抗中的关键调控机制。

2. **文献名称**: *"Role of IRS-1 in Breast Cancer Progression via PI3K/Akt Pathway"*

**作者**: Lee, H. et al.

**摘要**: 通过免疫沉淀和免疫荧光技术结合Ab-312抗体，研究发现IRS-1的过表达通过激活PI3K/Akt通路促进乳腺癌细胞增殖，为靶向IRS-1的癌症治疗提供了实验依据。

3. **文献名称**: *"IRS-1 Knockout Mice Reveal Adipose-Specific Metabolic Dysregulation"*

**作者**: Zhang, R. et al.

**摘要**: 利用IRS-1 (Ab-312)抗体检测脂肪组织中IRS-1蛋白水平，发现IRS-1缺失导致脂肪细胞分化异常和脂质代谢紊乱，强调了其在肥胖相关代谢综合征中的作用。

4. **文献名称**: *"Antibody Validation for IRS-1 Detection in Murine Models"*

**作者**: Patel, S. et al.

**摘要**: 本研究系统验证了Ab-312抗体在小鼠组织中的特异性，确认其适用于检测IRS-1的磷酸化及总蛋白表达，为胰岛素信号通路研究提供了可靠工具。

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**提示**：实际文献需通过PubMed、Google Scholar等平台，以关键词“IRS-1 antibody Ab-312”或“IRS-1 phosphorylation”检索，并筛选涉及该抗体具体应用的研究。部分经典文献可能来自抗体供应商的技术文档(如Cell Signaling Technology产品说明)。

背景信息

The IRS-1 (Ab-312) antibody is a widely used tool in metabolic and oncogenic research, targeting Insulin Receptor Substrate 1 (IRS-1), a critical signaling adapter protein in insulin and insulin-like growth factor (IGF) pathways. IRS-1 contains multiple tyrosine phosphorylation sites and serves as a docking platform for downstream effectors like PI3K and Grb2. regulating glucose uptake, cell growth, and survival. Dysregulation of IRS-1 is linked to insulin resistance, type 2 diabetes, and cancer progression. The Ab-312 antibody, often raised against a C-terminal epitope of human IRS-1. enables detection of IRS-1 expression and post-translational modifications (e.g., phosphorylation at Ser312) via techniques like Western blotting, immunoprecipitation, and immunofluorescence. Its specificity helps elucidate IRS-1’s role in insulin signaling crosstalk with other pathways (e.g., mTOR, MAPK) and its tissue-specific functions. Researchers employ this antibody to study metabolic disorders, tumorigenesis, and therapeutic responses, particularly in contexts where IRS-1 expression or activity is perturbed by mutations, oxidative stress, or hormonal imbalances. Validation in knockout models or phosphorylation-specific assays is recommended to ensure experimental accuracy.

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