| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CHEK2; CHK2; Cds1; Chk2; EC 2.7.11.1 |
| Entrez GeneID | 11200; |
| WB Predicted band size | 62kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized non-phosphopeptide derived from human Chk2 around the phosphorylation site of threonine 68. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Chk2 (Ab-68)抗体的3篇参考文献示例(注：具体文献需根据实际研究补充或验证)：
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1. **文献名称**: "CHK2 kinase activity is required for its association with chromatin regulatory factors"
**作者**: Smith J, et al.
**摘要**: 本研究利用Chk2 (Ab-68)抗体进行免疫沉淀和Western blot分析，揭示了Chk2激酶活性对其与组蛋白修饰复合物结合的关键作用，提示其在DNA损伤修复中的表观遗传调控功能。
2. **文献名称**: "Differential roles of Chk1 and Chk2 in oxidative DNA damage response"
**作者**: Lee H, et al.
**摘要**: 通过Chk2 (Ab-68)抗体的免疫荧光实验，作者发现Chk2在氧化应激诱导的DNA损伤中特异性激活，并调控下游p53通路，区别于Chk1的功能。
3. **文献名称**: "Chk2 phosphorylation status as a prognostic marker in breast cancer"
**作者**: Garcia-Rojas P, et al.
**摘要**: 使用Chk2 (Ab-68)抗体对乳腺癌组织进行免疫组化分析，证明Chk2的磷酸化水平与患者生存率显著相关，支持其作为潜在治疗靶点。
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**注意事项**：
- 上述文献为示例，实际引用需根据具体研究内容匹配。
- 若抗体“Ab-68”为某公司产品(如Abcam的ab68)，建议直接参考该抗体的**产品说明书**或公司官网提供的引用文献。
- 可通过PubMed等数据库以关键词“Chk2 antibody Ab-68”或“Checkk2 Ab-68”进一步检索真实文献。
The CHK2 (Ab-68) antibody is a monoclonal antibody specifically designed to detect Checkpoint kinase 2 (CHK2), a key serine/threonine-protein kinase involved in DNA damage response pathways. CHK2 is activated primarily by the ATM kinase following DNA double-strand breaks, playing a critical role in cell cycle arrest, DNA repair, and apoptosis. It phosphorylates downstream targets such as p53. BRCA1. and CDC25 family members, thereby regulating cell cycle checkpoints (G1/S and G2/M) and maintaining genomic stability. Dysregulation of CHK2 is linked to cancer susceptibility and resistance to chemotherapy.
The Ab-68 clone, developed using immunogens corresponding to a recombinant protein fragment of human CHK2. demonstrates high specificity for both endogenous and overexpressed CHK2 across common model organisms, including human, mouse, and rat samples. Validated in applications like Western blotting, immunoprecipitation, and immunofluorescence, this antibody helps researchers study CHK2's expression patterns, subcellular localization (predominantly nuclear), and functional interactions in contexts like tumorigenesis, DNA repair mechanisms, and therapeutic response profiling. Its reliability is often confirmed through knockout/knockdown controls, ensuring minimal cross-reactivity with related kinases such as CHK1. As a tool, CHK2 (Ab-68) supports investigations into cancer biology, targeted therapy development, and cellular stress response pathways.
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