| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | cAMP responsive element binding protein 1; cAMP-response element binding protein; CREB-1; CREB1; |
| Entrez GeneID | 1385; |
| WB Predicted band size | 43kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized non-phosphopeptide derived from human CREB around the phosphorylation site of serine 129 or serine 133(S-R-R-P-S). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及CREB (phospho-Ser133. Ab-129/133)抗体的经典文献摘要(内容虚构，仅供示例参考)：
1. **文献名称**: "CREB phosphorylation and survival of neurons"
**作者**: Ginty DD, Greenberg ME
**摘要**: 研究神经元存活中CREB磷酸化的作用，使用CREB (Ab-129/133)抗体检测神经生长因子刺激后Ser133位点磷酸化，证明其与神经元存活信号通路关联。
2. **文献名称**: "CREB function in long-term memory formation"
**作者**: Silva AJ, et al.
**摘要**: 通过CREB (Ab-129/133)抗体检测小鼠海马体CREB的Ser133磷酸化水平，发现其在小鼠学习记忆任务中显著上调，提示磷酸化CREB是突触可塑性的关键分子。
3. **文献名称**: "Transcriptional regulation by cAMP-responsive CREB"
**作者**: Lonze BE, Ginty DD
**摘要**: 利用CREB (Ab-129/133)抗体进行免疫印迹和免疫组化分析，揭示CREB磷酸化在cAMP信号通路中的动态变化及其对靶基因转录的调控机制。
*注：以上文献信息为示例性虚构，实际引用需查询具体数据库(如PubMed)并核实抗体应用细节。*
The CREB (cAMP Response Element-Binding Protein) antibody targeting phosphorylated residues at Serine 129/133 (Ab-129/133) is a critical tool for studying CREB activation in cellular signaling pathways. CREB is a transcription factor that regulates gene expression in response to stimuli such as hormones, growth factors, and neurotransmitters. Its activity is tightly controlled by phosphorylation at specific residues, including Ser133. a key site for its transcriptional activation. Phosphorylation at Ser133 promotes CREB's interaction with coactivators like CBP/p300. enabling transcription of target genes involved in processes like memory formation, neuronal survival, and metabolism. Some studies also suggest phosphorylation at Ser129 may modulate CREB activity or stability, though its role is less defined.
The CREB (Ab-129/133) antibody specifically detects dual phosphorylation at these residues, serving as a marker for activated CREB in response to pathways like cAMP/PKA, MAPK, or Ca²⁺/calmodulin kinases. It is widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to investigate CREB's role in diseases like cancer, neurological disorders, and metabolic syndromes. Validation often includes knockout controls or phosphatase treatments to confirm specificity. Researchers rely on this antibody to explore CREB dynamics in cellular models, tissue samples, or behavioral studies, linking phosphorylation events to functional outcomes in health and disease.
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