| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CHEK1; Chk1; kinase Chk1; |
| Entrez GeneID | 1111; |
| WB Predicted band size | 56kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized non-phosphopeptide derived from human Chk1 around the phosphorylation site of Serine 280. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Chk1 (Ab-280)抗体的3篇代表性文献(注：具体抗体编号可能与文献中描述略有差异，需结合实验背景确认)：
1. **文献名称**：*"Phosphorylation of Chk1 by ATM- and Rad3-related (ATR) in Xenopus egg extracts requires binding of ATRIP to ATR"*
**作者**：Guo Z. et al.
**摘要**：该研究利用Chk1 (Ab-280)抗体检测Chk1在Ser280位点的磷酸化，揭示了ATR激酶通过ATRIP适配蛋白激活Chk1的分子机制，强调该磷酸化事件在DNA损伤检查点调控中的关键作用。
2. **文献名称**：*"Distinct roles of Chk1 and Chk2 in regulating Cdc25A during DNA replication stress"*
**作者**：Mailand N. et al.
**摘要**：通过Chk1 (Ab-280)抗体进行免疫印迹分析，作者发现Chk1(而非Chk2)在DNA复制压力下特异性磷酸化Cdc25A，导致细胞周期停滞，表明Chk1的Ser280磷酸化是其功能活性的重要标志。
3. **文献名称**：*"Targeting Chk1 in p53-deficient triple-negative breast cancer"*
**作者**：King C. et al.
**摘要**：研究使用Chk1 (Ab-280)抗体评估肿瘤组织中Chk1激活状态，证明抑制Chk1磷酸化可增强p53缺陷型三阴性乳腺癌对化疗的敏感性，为靶向治疗提供了实验依据。
**注意**：不同厂商对同一抗体的命名可能不同(如Ab-280可能对应Ser280磷酸化抗体)，建议结合具体产品说明书或文献方法部分确认抗体特异性。若需更精准的文献，可提供抗体生产商或靶向修饰位点(如Ser280、Thr286等)进一步筛选。
Chk1 (Checkpoint Kinase 1) is a serine/threonine kinase critical for maintaining genomic stability by regulating cell cycle checkpoints and DNA damage response. It is activated primarily in response to replication stress or DNA damage, mediated via phosphorylation by upstream sensors like ATR (Ataxia Telangiectasia and Rad3-related kinase). Once activated, Chk1 phosphorylates downstream targets, including Cdc25 phosphatases, to halt cell cycle progression, allowing time for DNA repair or triggering apoptosis if damage is irreparable. This pathway is particularly important in S and G2/M phases, safeguarding against replication errors or mitotic catastrophe.
The Chk1 (Ab-280) antibody is a specific reagent designed to detect endogenous levels of total Chk1 protein, often targeting a conserved region (e.g., around amino acid 280) to ensure cross-reactivity across species like human, mouse, and rat. It is widely used in Western blotting, immunofluorescence, and immunoprecipitation to study Chk1 expression, localization, and interactions under conditions of genotoxic stress, such as UV radiation or chemotherapeutic agents. Researchers employ this antibody to explore Chk1's role in cancer biology, particularly in tumor cell survival, chemoresistance, and synergy with targeted therapies. Its specificity and reliability make it a key tool in dissecting DNA damage signaling mechanisms and validating Chk1 as a therapeutic target.
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