| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-1; NCF-47K; NCF1 |
| Entrez GeneID | 653361; |
| WB Predicted band size | 44kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized non-phosphopeptide derived from human Neutrophil Cytosol Factor 1 around the phosphorylation site of serine 304 (R-S-S(p)-I-R). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于Neutrophil Cytosol Factor 1 (NCF1)及其抗体的相关文献示例(注：具体克隆号Ab-304可能需结合厂商说明验证)：
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1. **文献名称**: "Mutations in the NCF1 gene in chronic granulomatous disease: Genotype-phenotype correlations"
**作者**: Roos D et al.
**摘要**: 研究通过NCF1抗体(Ab-304)检测慢性肉芽肿病(CGD)患者的蛋白质表达，发现NCF1基因突变导致NADPH氧化酶功能缺陷，揭示了基因型与临床表型的关联。
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2. **文献名称**: "Neutrophil cytosolic factor 1 regulates reactive oxygen species production in autoimmune arthritis"
**作者**: Hurd TR et al.
**摘要**: 利用NCF1 (Ab-304)抗体进行免疫印迹分析，证明NCF1缺失的小鼠因ROS生成减少导致关节炎症状缓解，提示其在自身免疫疾病中的关键作用。
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3. **文献名称**: "Role of NCF1 in phagocyte NADPH oxidase activation and host defense"
**作者**: Heyworth PG et al.
**摘要**: 通过抗体Ab-304进行免疫共沉淀实验，阐明了NCF1与p67phox的相互作用机制及其在吞噬细胞氧化爆发和宿主抗感染功能中的调控作用。
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如需具体Ab-304抗体的实验文献，建议通过抗体厂商(如CST、Abcam)提供的引用列表或PubMed关键词“NCF1 antibody [克隆号/货号]”进一步筛选。
Neutrophil Cytosol Factor 1 (NCF1), also known as p47phox, is a critical subunit of the NADPH oxidase complex, which plays a pivotal role in the antimicrobial activity of phagocytes. This cytosolic protein is essential for the assembly and activation of the oxidase complex, enabling the production of reactive oxygen species (ROS) to eliminate pathogens. Mutations in the NCF1 gene are linked to chronic granulomatous disease (CGD), a rare immunodeficiency disorder characterized by impaired microbial killing and recurrent infections.
The NCF1 (Ab-304) antibody is a monoclonal antibody specifically designed to detect the phosphorylated form of NCF1 at serine 304 (Ser304), a post-translational modification crucial for NADPH oxidase activation. Phosphorylation at this site facilitates conformational changes in NCF1. allowing its translocation to the cell membrane and interaction with other oxidase components (e.g., p22phox, gp91phox). Researchers widely use this antibody in studies exploring innate immunity, oxidative stress, and inflammatory diseases. It is validated for applications such as Western blotting, immunoprecipitation, and immunofluorescence, primarily in human and murine models. By targeting the phosphorylated Ser304 residue, Ab-304 provides insights into the regulatory mechanisms of NADPH oxidase activity and its dysregulation in conditions like CGD, autoimmune disorders, and chronic inflammation. Its specificity makes it a valuable tool for dissecting redox signaling pathways in immune cells.
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