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Rabbit Polyclonal RFA2(Ab-21) Antibody

  • 中文名: RFA2 (Ab-21)抗体
  • 别    名: REPA2; RF-A; RP-A; RPA2; RPA32
货号: IPDX41666
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesREPA2; RF-A; RP-A; RPA2; RPA32
Entrez GeneID6118;
WB Predicted band size32kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenSynthesized non-phosphopeptide derived from human RFA2 around the phosphorylation site of threonine 21 (G-Y-T(p)-Q-S).
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于RFA2(Ab-21)抗体的示例参考文献(内容为虚构,仅作格式参考):

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1. **标题**: *RFA2 modulates replication fork stability in yeast through interaction with DNA polymerase ε*

**作者**: Thompson L, et al.

**摘要**: 本研究利用RFA2 (Ab-21)抗体通过免疫共沉淀(Co-IP)和Western blot技术,揭示了酵母RFA2蛋白与DNA聚合酶ε的相互作用,证明其在维持复制叉稳定性中的关键作用。结果显示RFA2缺失导致DNA损伤敏感性增加。

2. **标题**: *Role of RFA2 in homologous recombination repair after ionizing radiation*

**作者**: Chen H, et al.

**摘要**: 通过RFA2 (Ab-21)抗体的染色质免疫沉淀(ChIP)分析,发现RFA2在电离辐射后快速募集到DNA双链断裂位点,并促进Rad51介导的同源重组修复。实验表明RFA2缺失会显著延缓修复进程。

3. **标题**: *RFA2 as a biomarker for genomic instability in cancer cells*

**作者**: Martinez R, et al.

**摘要**: 使用RFA2 (Ab-21)抗体进行免疫组化分析,发现多种癌细胞中RFA2表达水平与染色体异常呈负相关。研究提示RFA2可能作为评估肿瘤基因组不稳定的潜在标志物。

4. **标题**: *Structural insights into RPA complex assembly via RFA2 C-terminal domain*

**作者**: Park J, et al.

**摘要**: 通过RFA2 (Ab-21)抗体阻断实验及冷冻电镜技术,解析了RPA复合体中RFA2的C端结构域对DNA结合能力的调控机制,为靶向DNA修复通路的设计提供依据。

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**注意**:以上文献为模拟内容,实际引用需通过PubMed、Google Scholar等平台检索真实研究(关键词:RFA2 antibody, replication protein A, DNA repair)。若Ab-21为某公司抗体产品(如Santa Cruz产品编号),可查阅其官网提供的引用文献列表。

背景信息

The RPA2 (Ab-21) antibody is a well-characterized reagent designed to target Replication Protein A2 (RPA2), a critical subunit of the Replication Protein A (RPA) complex. RPA, a single-stranded DNA (ssDNA)-binding protein, plays essential roles in DNA replication, repair, and recombination. The RPA complex, composed of RPA1 (70 kDa), RPA2 (32 kDa), and RPA3 (14 kDa), stabilizes ssDNA during replication and participates in DNA damage response pathways, including homologous recombination and nucleotide excision repair. RPA2 contains multiple phosphorylation sites that regulate its function in cell cycle checkpoints and DNA damage signaling. The RFA2 (Ab-21) antibody, often generated against a specific epitope or recombinant fragment of human RPA2. is widely used in research to study DNA replication stress, genomic instability, and cancer biology. It enables detection of RPA2 expression, phosphorylation status (e.g., at Ser4/Ser8), and localization via techniques like Western blotting, immunofluorescence, and immunoprecipitation. This antibody has been instrumental in exploring RPA2's role in chemotherapy/radiation responses, replication fork dynamics, and diseases linked to DNA repair defects, such as cancers and premature aging syndromes. Its specificity and reliability make it a key tool for dissecting mechanisms underlying genome maintenance and therapeutic resistance.

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