Rabbit Polyclonal PKA-R2β(Ab-113) Antibody

The PKA-R2β (Ab-113) antibody is a selective monoclonal or polyclonal antibody (depending on the source) designed to target the regulatory subunit RIIβ (PRKAR2B) of cAMP-dependent protein kinase A (PKA), a critical enzyme in cellular signaling. PKA consists of two regulatory (R) and two catalytic (C) subunits, with RIIβ being one of four regulatory isoforms (RIα, RIβ, RIIα, RIIβ). The RIIβ subunit is notably expressed in adipose tissue, brain, and other organs, playing a role in metabolic regulation, lipolysis, and neuronal signaling. This antibody is commonly used to study PKA-RIIβ expression, localization, and function in various biological contexts, including obesity, diabetes, cancer, and neurological disorders. It is validated for applications such as Western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF) in human, mouse, or rat samples. By detecting RIIβ, researchers can explore its role in cAMP-PKA signaling pathways, particularly its interaction with A-kinase anchoring proteins (AKAPs) that compartmentalize PKA activity. Aberrant RIIβ expression has been linked to metabolic syndromes and tumors, making this antibody a valuable tool for mechanistic and therapeutic research. Specificity is often confirmed using knockout controls or isoform-specific assays to distinguish RIIβ from other PKA subunits.