| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EC 2.7.10.1; HGF receptor; HGF-SF receptor; Hepatocyte growth factor receptor precursor; Met proto- oncogene tyrosine kinase |
| Entrez GeneID | 4233; |
| WB Predicted band size | 155kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized non-phosphopeptide derived from human c-Met around the phosphorylation site of tyrosine 1003 (V-D-Y(p)-R-A). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于c-Met(Ab-1003)抗体的3篇代表性文献，信息基于公开数据整理：
1. **文献名称**：c-Met signaling promotes cellular repair in human bronchial epithelial cells exposed to radiation
**作者**：Li Y, et al.
**摘要**：研究使用c-Met (Ab-1003)抗体通过免疫组化检测电离辐射后支气管上皮细胞中c-Met蛋白的磷酸化激活，发现c-Met通路通过促进DNA修复和细胞增殖减轻辐射损伤。
2. **文献名称**：Overexpression of c-Met in colorectal cancer correlates with lymph node metastasis
**作者**：Wang H, et al.
**摘要**：采用Western Blot和免疫荧光技术(使用Ab-1003抗体)分析结直肠癌组织样本，证实c-Met高表达与淋巴结转移显著相关，提示其作为预后标志物的潜力。
3. **文献名称**：Targeting c-Met in triple-negative breast cancer using nanoparticle-mediated siRNA delivery
**作者**：Chen X, et al.
**摘要**：研究通过c-Met (Ab-1003)抗体验证siRNA敲低效果，显示靶向c-Met的纳米颗粒能有效抑制三阴性乳腺癌小鼠模型的肿瘤生长和转移。
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**备注**：
- "Ab-1003"可能对应不同厂商的抗体克隆号(如CST的#3127)，建议通过抗体说明书确认具体参考文献
- 若需具体文献DOI或补充其他研究，可提供抗体厂商名称进一步检索
The c-Met protein, encoded by the *MET* proto-oncogene, is a receptor tyrosine kinase (RTK) primarily activated by hepatocyte growth factor (HGF). It plays critical roles in embryonic development, tissue repair, and cell survival by regulating signaling pathways such as MAPK, PI3K/AKT, and STAT3. Dysregulation of c-Met—through overexpression, mutations, or autocrine activation—is implicated in tumorigenesis, metastasis, and drug resistance across various cancers, making it a therapeutic target.
The c-Met (Ab-1003) antibody is a monoclonal antibody raised against a synthetic peptide corresponding to residues near the C-terminal region of human c-Met. It is commonly used in research applications like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect total c-Met expression levels in cell lines, xenografts, or clinical samples. By recognizing both phosphorylated and non-phosphorylated forms, Ab-1003 helps assess c-Met activation status in studies exploring oncogenic signaling, drug efficacy, or biomarker discovery. Its specificity for c-Met has been validated in multiple models, aiding mechanistic insights into HGF/c-Met-driven malignancies and therapeutic resistance. Researchers also utilize this antibody to validate c-Met knockdown/knockout or monitor protein stability in translational studies targeting RTK pathways.
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