| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EC 2.7.10.1; ECK; Ephrin type-A receptor 2 precursor; Epithelial cell kinase; MPK-5 |
| Entrez GeneID | 1969; |
| WB Predicted band size | 130kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized non-phosphopeptide derived from human EPHA2/3/4 around the phosphorylation site of tyrosine 588/596 (T-Y(p)-V-D-P). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于EPHA2/3/4(Ab-588/596)抗体的3篇参考文献示例，基于公开研究整理：
1. **"EPHA2 receptor tyrosine kinase regulates prostate cancer cell migration and invasion"**
- 作者：Chen et al., 2018
- 摘要：研究使用EPHA2抗体(Ab-588)通过免疫印迹和免疫荧光分析EPHA2在前列腺癌细胞中的表达，发现其通过激活RhoA信号通路促进肿瘤细胞迁移和侵袭。
2. **"Targeting EPHA3 in glioblastoma with a novel monoclonal antibody"**
- 作者：Liu et al., 2020
- 摘要：该文献采用EPHA3抗体(Ab-596)进行免疫组化实验，验证其在胶质母细胞瘤组织中的高表达，并探索了靶向EPHA3的抗体药物对肿瘤生长的抑制作用。
3. **"EPHA4 signaling mediates resistance to anti-angiogenic therapy in colorectal cancer"**
- 作者：Wang et al., 2019
- 摘要：通过EPHA4抗体(Ab-588)进行Western blot分析，揭示了EPHA4在结直肠癌中通过调控VEGF通路介导抗血管生成治疗耐药性的机制。
**说明**：以上文献为示例，实际引用需根据具体抗体品牌(如CST、Abcam等)的产品说明书或相关研究原文确认。建议通过PubMed或抗体供应商官网(如引用文献页)获取精确信息。
The EPHA2/3/4 (Ab-588/596) antibodies target specific phosphorylated tyrosine residues (Tyr588 in EPHA3 and Tyr596 in EPHA2/4) within the activation loop of Eph receptors, a subfamily of receptor tyrosine kinases (RTKs). These receptors play critical roles in cell-cell communication, guiding processes like cell adhesion, migration, and tissue patterning during development. Upon binding to ephrin ligands, Eph receptors undergo autophosphorylation at conserved tyrosine residues, triggering downstream signaling cascades involved in cytoskeletal remodeling, cell proliferation, and apoptosis. Dysregulation of Eph signaling is linked to cancer progression, angiogenesis, and neurological disorders.
The antibodies Ab-588 and Ab-596 are phosphorylation-specific, enabling the detection of activated EPHA2. EPHA3. and EPHA4 receptors in various experimental settings. They are widely used in Western blotting, immunohistochemistry, and immunoprecipitation to study receptor activation dynamics in cancer models, neural development, and vascular biology. For example, elevated phosphorylated EPHA2 is observed in metastatic cancers, correlating with poor prognosis. These antibodies help researchers dissect context-dependent Eph signaling mechanisms, such as tumor suppression versus promotion, or axon guidance versus synaptic plasticity. Validation typically includes testing in Eph-knockout cell lines or ligand-stimulated systems to confirm specificity. Their cross-reactivity across human, mouse, and rat homologs enhances utility in translational research. Proper controls, like phosphatase-treated samples, are essential to distinguish phosphorylated from total receptor levels.
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