Rabbit Polyclonal DAPK3(Ab-265) Antibody

The DAPK3 (Ab-265) antibody is a monoclonal antibody specifically designed to detect Death-Associated Protein Kinase 3 (DAPK3), a serine/threonine kinase belonging to the DAPK family. DAPK3. also known as ZIP kinase (ZIPk), plays critical roles in apoptosis, autophagy, and cytoskeletal reorganization. It is involved in stress-induced cell death pathways and interacts with signaling molecules like p53. TP53. and myosin phosphatase targeting subunit 1 (MYPT1) to regulate cell cycle progression and anoikis. The antibody, often generated against a recombinant protein fragment corresponding to amino acids within the catalytic domain of human DAPK3. is widely used in research applications such as Western blotting, immunoprecipitation, and immunofluorescence to study endogenous DAPK3 expression and localization. Its specificity for DAPK3 helps distinguish it from other DAPK family members (e.g., DAPK1. DAPK2), enabling precise investigation of its unique functions. DAPK3 dysregulation has been implicated in cancer, neurodegenerative diseases, and cardiovascular disorders, making this antibody a valuable tool for exploring disease mechanisms. Studies using Ab-265 have highlighted DAPK3's tumor-suppressive role in certain cancers and its potential as a therapeutic target. Validation typically includes testing in knockout cell lines to confirm absence of cross-reactivity.