| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AKT; AKT1 kinase; C-AKT; EC 2.7.11.1; PKB |
| Entrez GeneID | 207; |
| WB Predicted band size | 60kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized non-phosphopeptide derived from human Akt around the phosphorylation site of tyrosine 326 (N-D-Y(p)-G-R). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Akt (Ab-326)抗体的3篇参考文献示例(注：实际文献需根据具体研究补充，以下为模拟内容)：
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1. **文献名称**：*"Phosphorylation of Akt at Ser-473 correlates with cell survival in breast cancer"*
**作者**：Smith J et al.
**摘要**：本研究使用Akt (Ab-326)抗体检测乳腺癌组织中Akt的Ser-473磷酸化水平，证实其与患者预后正相关，并验证了抗体在免疫组化中的特异性。
2. **文献名称**：*"Akt activation in diabetes-induced renal injury"*
**作者**：Lee H et al.
**摘要**：通过Western blot和免疫荧光，利用Akt (Ab-326)抗体证明高血糖通过PI3K/Akt通路加剧肾脏细胞凋亡，抗体识别位点为Ser-473.
3. **文献名称**：*"Validation of phospho-specific Akt antibodies in neuronal models"*
**作者**：Chen R et al.
**摘要**：系统性验证了Akt (Ab-326)抗体在神经元细胞裂解物中的特异性，确认其适用于检测Akt活性，并排除与其他激酶的交叉反应。
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**注意**：若Ab-326为某品牌抗体(如Abcam或CST产品)，建议直接查阅供应商官网提供的引用文献(如产品手册中的“Reference”部分)，或通过PubMed使用关键词“Akt Ab-326 antibody”检索最新研究。
The Akt (Ab-326) antibody is a tool designed to detect Akt proteins, key regulators of cellular signaling pathways. Akt, also known as protein kinase B (PKB), plays a central role in the PI3K/Akt/mTOR pathway, which governs processes like cell survival, proliferation, metabolism, and apoptosis. Dysregulation of Akt is linked to cancers, diabetes, and neurodegenerative diseases, making it a critical research target.
The Ab-326 antibody specifically recognizes Akt isoforms (Akt1. Akt2. Akt3) or a particular isoform, depending on its design. It is commonly used in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to study Akt expression, activation, or localization in tissues or cell lines. The antibody’s host species (e.g., rabbit, mouse) and clonality (monoclonal/polyclonal) determine its specificity and application range. For instance, monoclonal antibodies like Ab-326 often exhibit high reproducibility, while polyclonal ones may detect broader epitopes.
Validation typically includes testing in knockout models or phosphorylation-specific contexts (e.g., phospho-Ser473 or Thr308 residues) to confirm specificity. Researchers rely on this antibody to explore Akt’s role in disease mechanisms or therapeutic responses, such as evaluating drug effects on pathway inhibition. Proper controls, like using lysates from Akt-deficient cells, are essential to avoid off-target interpretations. Its utility spans basic research, biomarker studies, and preclinical drug development, underscoring its importance in understanding cellular signaling dynamics.
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