Rabbit Polyclonal MEK1(Ab-221) Antibody

The MEK1 (Ab-221) antibody is a widely used tool in molecular biology research, specifically targeting the Mitogen-Activated Protein Kinase Kinase 1 (MEK1), a critical component of the MAPK/ERK signaling pathway. MEK1. also known as MAP2K1. functions as a dual-specificity kinase that activates ERK1/2 through phosphorylation, playing a central role in regulating cell proliferation, differentiation, and survival. Dysregulation of MEK1 is frequently associated with cancers and developmental disorders. The Ab-221 clone recognizes endogenous MEK1 proteins across multiple species, including human, mouse, and rat, making it versatile for cross-species studies. It is commonly employed in applications such as Western blotting, immunoprecipitation, and immunohistochemistry to assess MEK1 expression levels or activation status in cellular models. The antibody typically detects both phosphorylated and non-phosphorylated forms of MEK1. though this may vary depending on experimental conditions. Validation often includes testing in knockout cell lines to confirm specificity. Researchers frequently use MEK1 (Ab-221) in studies investigating RAS/RAF/MEK/ERK pathway dynamics, drug resistance mechanisms, and targeted therapy development, particularly in oncology. Its reliability in detecting MEK1 has made it a staple in signal transduction research, helping to elucidate the protein's role in health and disease.