Rabbit Polyclonal Src(Ab-529) Antibody

Src (Ab-529) is a monoclonal antibody developed to target the c-Src protein, a non-receptor tyrosine kinase encoded by the SRC proto-oncogene. c-Src plays a critical role in regulating cellular signaling pathways involved in proliferation, differentiation, adhesion, and migration. Dysregulation of c-Src activity is linked to cancer progression, metastasis, and resistance to therapies, making it a key focus in oncological research. The Ab-529 antibody specifically recognizes the C-terminal region of c-Src, which contains critical regulatory domains, including the SH2 and SH3 regions, responsible for protein-protein interactions and autoinhibition. By binding to these domains, Ab-529 can inhibit c-Src kinase activity, making it a valuable tool for studying Src-mediated signaling cascades and their therapeutic targeting. This antibody has been widely used in applications such as Western blotting, immunoprecipitation, and immunohistochemistry to detect c-Src expression and activation in cell lines, tumor tissues, and preclinical models. Its specificity for c-Src over other Src-family kinases (e.g., Yes, Fyn) enhances its utility in dissecting the distinct roles of these closely related proteins. Research involving Ab-529 has contributed to insights into c-Src's involvement in pathways like EGFR, FAK, and PI3K/AKT, as well as its cross-talk with tumor microenvironment components. Developed and validated in rigorous assays, Src (Ab-529) remains a cornerstone reagent for both basic and translational studies in cancer biology.