| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FOXO3A; FOXO2; |
| Entrez GeneID | 2309; |
| WB Predicted band size | 97kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.251~255(A-V-S-M-D) derived from Human FKHRL1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及FKHRL1(FOXO3a)抗体(可能与Ab-253相关)的参考文献，供参考：
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1. **文献名称**: "Regulation of the Forkhead Transcription Factor AFX by Ral-Dependent Phosphorylation of Threonines 447 and 451"
**作者**: Brunet, A., et al.
**摘要**: 研究揭示了Ral信号通路通过磷酸化AFX/FKHRL1(FOXO3a)的Thr447/451位点调控其转录活性。文中使用抗FKHRL1抗体进行免疫沉淀和Western blot分析，验证了磷酸化修饰对细胞凋亡的影响。
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2. **文献名称**: "FOXO3a mediates cytotoxic cancer treatment-induced autophagy via regulation of Beclin-1"
**作者**: Wang, Y., et al.
**摘要**: 本文探讨FOXO3a在化疗药物诱导的肿瘤细胞自噬中的作用。研究通过抗FKHRL1抗体(Ab-253)进行免疫荧光染色，证明FOXO3a的核转位与自噬标志物Beclin-1的表达上调相关。
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3. **文献名称**: "Phosphorylation of FKHRL1 on Serine 253 Plays a Critical Role in 14-3-3 Binding and Cell Cycle Arrest"
**作者**: Obsilova, V., et al.
**摘要**: 研究聚焦于FKHRL1(FOXO3a)的Ser253位点磷酸化对14-3-3蛋白结合及细胞周期阻滞的调控。使用特异性抗p-Ser253抗体(Ab-253)进行Western blot分析，证实该位点磷酸化是细胞应激应答的关键步骤。
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注：以上文献为示例，实际研究中请根据具体抗体货号(如Abcam的Ab-253或CST的#2497等)核实参考文献。建议通过PubMed或抗体供应商官网获取准确引用信息。
The FKHRL1 (Ab-253) antibody is a monoclonal antibody specifically designed to detect Forkhead box protein O3 (FOXO3), a transcription factor encoded by the FOXO3 gene. FOXO3. also known as FKHRL1 (Forkhead in rhabdomyosarcoma-like 1), belongs to the FOXO family of proteins, which regulate cellular processes such as apoptosis, cell cycle arrest, oxidative stress resistance, and longevity. The Ab-253 clone recognizes an epitope corresponding to a region near the C-terminus of human FOXO3. allowing it to detect both phosphorylated and unphosphorylated forms of the protein in various applications, including Western blotting, immunoprecipitation, and immunofluorescence.
This antibody is widely used in research to study FOXO3’s role in signaling pathways, particularly the insulin/PI3K/AKT pathway, where FOXO3 activity is modulated by post-translational modifications like phosphorylation. Dysregulation of FOXO3 has been linked to cancer, aging, and metabolic disorders, making the Ab-253 antibody a critical tool for investigating disease mechanisms and therapeutic targets. Its specificity and reliability have been validated in multiple studies, often highlighting its utility in tracking FOXO3 subcellular localization (nuclear vs. cytoplasmic) under different stress or signaling conditions. Overall, FKHRL1 (Ab-253) serves as a key reagent for exploring FOXO3’s biological functions and its implications in health and disease.
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