Rabbit Polyclonal eNOS(Ab-1177) Antibody

The endothelial nitric oxide synthase (eNOS) antibody, particularly the eNOS(Ab-1177) clone, is a widely used tool in biomedical research to study the expression and regulation of eNOS, a critical enzyme responsible for nitric oxide (NO) production in vascular endothelial cells. eNOS, encoded by the *NOS3* gene, catalyzes the conversion of L-arginine to L-citrulline and NO, a key signaling molecule mediating vasodilation, vascular homeostasis, and anti-inflammatory responses. The Ab-1177 antibody specifically targets the phosphorylated form of eNOS at serine residue 1177 (p-eNOS Ser1177), a post-translational modification associated with enzyme activation. Phosphorylation at this site, often induced by Akt kinase or other upstream signaling pathways (e.g., PI3K/Akt), enhances eNOS activity and NO release, playing a pivotal role in cardiovascular health and disease. Researchers employ this antibody in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess eNOS activation status in endothelial cells, tissues, or experimental models of hypertension, atherosclerosis, or diabetes. Its specificity for the phosphorylated form allows differentiation between basal and stimulated eNOS activity, aiding mechanistic studies of endothelial dysfunction. Validation typically includes testing in knockout models or phosphorylation-blocking assays to confirm target specificity.