Rabbit Polyclonal Vav(Ab-174) Antibody

The Vav family of proteins (Vav1. Vav2. Vav3) are guanine nucleotide exchange factors (GEFs) that activate Rho/Rac GTPases, playing critical roles in intracellular signaling pathways. Vav1. primarily expressed in hematopoietic cells, is essential for lymphocyte development, activation, and receptor-mediated signaling, such as T-cell receptor (TCR) and B-cell receptor (BCR) signaling. Vav2 and Vav3 are more ubiquitously expressed and regulate diverse processes, including cell adhesion, migration, and oncogenic transformation. The Vav(Ab-174) antibody, often referenced by clone number or catalog identifiers like sc-174. is a monoclonal antibody commonly used to detect Vav1 in human, mouse, or rat samples. It typically targets specific epitopes or post-translational modifications, such as phosphorylation sites (e.g., Tyr174 in activated Vav1), enabling studies of its activation status. This antibody is widely utilized in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate Vav1's role in immune cell signaling, cancer progression, or hematological disorders. Its specificity for Vav1. with minimal cross-reactivity to Vav2/Vav3. makes it valuable for dissecting isoform-specific functions in cellular responses. Research applications include analyzing downstream pathways, interactions with adaptor proteins (e.g., SLP-76), and pathological contexts like leukemia or autoimmune diseases where Vav dysregulation is implicated.