| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 70 kDa zeta-associated protein; SRK; Syk-related tyrosine kinase; ZA70; ZAP-70 |
| Entrez GeneID | 7535; |
| WB Predicted band size | 70kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.317~321 (S-P-Y-S-D) derived from Human ZAP70. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3条关于Zap-70抗体的虚构参考文献示例(仅供参考，实际文献需具体检索)：
---
1. **Title**: "ZAP-70 Expression as a Prognostic Marker in Chronic Lymphocytic Leukemia"
**Authors**: Smith JR, Lee H, et al.
**Summary**: 研究证明ZAP-70(Ab-319)抗体通过流式细胞术检测B-CLL患者ZAP-70蛋白表达水平，其高表达与IgHV未突变状态相关，可作为疾病进展和生存率差的独立预测因子。
2. **Title**: "Structural Insights into ZAP-70 Kinase Activation via TCR Signaling"
**Authors**: Chen L, Wang Y.
**Summary**: 利用ZAP-70(Ab-319)进行免疫共沉淀和Western blot分析，揭示T细胞受体(TCR)激活后ZAP-70的构象变化及磷酸化机制，阐明其在T细胞信号转导中的关键作用。
3. **Title**: "Comparative Evaluation of Commercial ZAP-70 Antibodies in Hematologic Malignancies"
**Authors**: Gonzalez M, Patel S.
**Summary**: 对比ZAP-70(Ab-319)与其他商业化抗体的特异性及灵敏度，验证其在骨髓瘤和淋巴瘤样本中的诊断一致性，推荐优化染色条件以提高临床检测准确性。
---
注：以上内容为模拟文献，如需真实文献建议通过PubMed、Google Scholar等平台以关键词 "ZAP-70 antibody" + "clinical significance" / "structure" / "CLL" 进行检索。
The Zap-70 (Ab-319) antibody is a widely used reagent for detecting ZAP-70 (zeta-chain-associated protein kinase 70), a critical tyrosine kinase in immune cell signaling. Expressed primarily in T cells and natural killer (NK) cells, ZAP-70 plays a pivotal role in T-cell receptor (TCR)-mediated signal transduction. Upon TCR activation, ZAP-70 is recruited to phosphorylated immunoreceptor tyrosine-based activation motifs (ITAMs) on the CD3ζ chain, where it becomes activated and initiates downstream signaling cascades essential for T-cell differentiation, proliferation, and immune response regulation. Dysregulation of ZAP-70 is linked to immunodeficiency disorders and hematologic malignancies, particularly chronic lymphocytic leukemia (CLL), where its expression correlates with disease progression and prognosis.
The Ab-319 clone is a monoclonal antibody developed to recognize specific epitopes of human ZAP-70. It is commonly employed in techniques like Western blotting, immunohistochemistry (IHC), and flow cytometry to assess ZAP-70 expression levels in research and diagnostic contexts. In CLL, ZAP-70 status (positive or negative) serves as a prognostic marker, with ZAP-70-positive cases often exhibiting more aggressive clinical behavior akin to unmutated immunoglobulin heavy-chain variable (IGHV) status. The antibody’s specificity and reliability make it valuable for studying ZAP-70’s role in immune dysfunction and oncogenesis, as well as for guiding therapeutic strategies in lymphoid malignancies.
×
