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Rabbit Polyclonal AANAT(N-term) Antibody

  • 中文名： AANAT (N-term)抗体
  • 别    名： Serotonin N-acetyltransferase, Serotonin acetylase, Aralkylamine N-acetyltransferase, AA-NAT, AANAT, SNAT
货号: IPDX34795
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesSerotonin N-acetyltransferase, Serotonin acetylase, Aralkylamine N-acetyltransferase, AA-NAT, AANAT, SNAT
Entrez GeneID15
WB Predicted band size23.3kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis AANAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-54 amino acids from the N-terminal region of human AANAT.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是3-4条关于 **AANAT (N-term)抗体** 的参考文献示例(注：文献为虚构示例，实际引用请通过学术数据库验证)：

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1. **文献名称**: *"Characterization of AANAT Expression in the Pineal Gland: Role of N-terminal Antibodies in Circadian Rhythm Studies"*

**作者**: Ganguly S, et al.

**摘要**: 本研究使用针对AANAT蛋白N端的特异性抗体，通过免疫印迹和免疫组化技术，揭示了AANAT在松果体中的昼夜节律性表达，并验证其与褪黑素合成的关联性。

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2. **文献名称**: *"Development and Application of a Polyclonal Antibody Targeting the N-terminal Domain of Arylalkylamine N-Acetyltransferase"*

**作者**: Roseboom PH, et al.

**摘要**: 文章报道了一种针对AANAT N端结构域的多克隆抗体的开发，通过免疫沉淀和Western blot验证其特异性，并应用于研究不同物种中AANAT的蛋白表达及调控机制。

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3. **文献名称**: *"Phosphorylation-Dependent Regulation of AANAT Activity: Insights from N-terminal Antibody-Based Assays"*

**作者**: Klein DC, et al.

**摘要**: 利用N端特异性抗体，研究AANAT的磷酸化修饰对其酶活性的调控，发现N端结构域的关键位点磷酸化显著影响其稳定性和昼夜节律功能。

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4. **文献名称**: *"Localization of AANAT in Retinal Photoreceptors Using N-terminal Targeted Immunohistochemistry"*

**作者**: Chaurasia SS, et al.

**摘要**: 通过N端抗体介导的免疫荧光技术，揭示AANAT在视网膜感光细胞中的亚细胞定位，并探讨其在非松果体组织中的潜在生理作用。

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建议通过 **PubMed** 或 **Google Scholar** 搜索关键词如 *"AANAT N-terminal antibody"* 或 *"Arylalkylamine N-acetyltransferase antibody characterization"* 获取真实文献。

背景信息

The AANAT (N-term) antibody is designed to target the N-terminal region of arylalkylamine N-acetyltransferase (AANAT), a critical enzyme in melatonin synthesis. AANAT catalyzes the conversion of serotonin to N-acetylserotonin, the precursor of melatonin, and is primarily expressed in the pineal gland and retina. Its activity is tightly regulated by circadian rhythms, light exposure, and post-translational modifications, making it pivotal in modulating sleep-wake cycles and seasonal biological processes. The N-terminal domain of AANAT is essential for protein stability, enzyme activity, and interactions with regulatory proteins like 14-3-3. which stabilize the enzyme in response to cyclic AMP signaling. Antibodies specific to the N-terminal region are valuable tools for studying AANAT expression, localization, and regulation in circadian biology, sleep disorders, and neuroendocrine research. They are commonly used in techniques such as Western blotting, immunohistochemistry, and immunocytochemistry to assess tissue-specific expression, circadian fluctuations, or pathological alterations in AANAT levels. Validated for reactivity across species like humans, mice, and rats, these antibodies help elucidate mechanisms underlying melatonin-related disorders, jet lag, and mood regulation. Proper controls, such as knockout validation, are recommended to confirm specificity due to potential cross-reactivity with structurally similar proteins.

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