| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Collagen alpha-1(VIII) chain, Endothelial collagen, Vastatin, COL8A1, C3orf7 |
| Entrez GeneID | 1295 |
| WB Predicted band size | 73.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This COL8A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-89 amino acids from the N-terminal region of human COL8A1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于COL8A1(N-term)抗体的3篇参考文献的简要信息：
1. **文献名称**：*Collagen VIII α1 Chain (COL8A1) Expression in Fuchs Endothelial Corneal Dystrophy*
**作者**：Jun AS, et al.
**摘要**：研究利用COL8A1(N-term)抗体通过免疫组化技术，揭示了Fuchs角膜内皮营养不良(FECD)患者角膜中COL8A1蛋白的异常沉积，表明其与疾病病理相关。
2. **文献名称**：*Role of Collagen VIII in Angiogenesis and Tumor Stroma Formation*
**作者**：Hou G, et al.
**摘要**：通过Western blot和免疫荧光实验(使用COL8A1 N-term抗体)，研究发现COL8A1在肿瘤血管生成和基质重塑中起调控作用，影响肿瘤微环境的结构完整性。
3. **文献名称**：*Extracellular Matrix Remodeling by COL8A1 in Diabetic Retinopathy*
**作者**：Meng H, et al.
**摘要**：该文献通过COL8A1(N-term)抗体检测糖尿病视网膜病变患者的视网膜组织，发现COL8A1表达上调与血管基底膜增厚及渗漏相关，提示其参与疾病进展。
(注：以上文献信息为模拟内容，实际引用需根据具体文献调整。)
The COL8A1 (N-term) antibody is a specific reagent designed to target the N-terminal region of collagen type VIII alpha 1 chain, a protein encoded by the COL8A1 gene. Collagen VIII, a short-chain collagen, is a key component of the extracellular matrix (ECM) and plays critical roles in tissue development, angiogenesis, and maintaining structural integrity. It is prominently expressed in vascular endothelial cells, corneal tissue, and certain epithelial layers, where it contributes to basement membrane organization and cell adhesion. COL8A1 has been implicated in pathologies such as Fuchs' endothelial corneal dystrophy and vascular remodeling disorders.
The antibody is commonly used in research applications like Western blotting, immunohistochemistry, and immunofluorescence to detect COL8A1 expression and localization in biological samples. Its specificity for the N-terminal domain allows researchers to distinguish COL8A1 from other collagen isoforms. Developed in hosts such as rabbits or mice, the antibody’s validation typically includes reactivity confirmation across species (e.g., human, mouse) and verification via knockdown or knockout controls. Its utility extends to studying ECM dynamics, tissue fibrosis, and disease mechanisms linked to collagen VIII dysregulation. Proper validation ensures reliability in experimental models, supporting its role in both basic and translational research.
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