Rabbit Polyclonal MAP4K1-T165 Antibody

The MAP4K1-T165 antibody is specifically designed to detect the phosphorylation of threonine 165 (T165) on mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), also known as hematopoietic progenitor kinase 1 (HPK1). MAP4K1 is a serine/threonine kinase belonging to the Ste20 kinase family, which plays a critical role in immune cell signaling, T-cell receptor (TCR) activation, and apoptosis regulation. Phosphorylation at T165 is a key post-translational modification linked to MAP4K1 activation, influencing downstream pathways such as the c-Jun N-terminal kinase (JNK) and nuclear factor kappa B (NF-κB) signaling cascades. This antibody is widely used in research to investigate MAP4K1's function in immune responses, particularly in contexts like autoimmune diseases, cancer immunotherapy, and T-cell exhaustion. By targeting the T165 site, it helps elucidate how MAP4K1 modulates immune cell activity, cytokine production, and tumor microenvironment interactions. Validated for applications like Western blotting, immunoprecipitation, and flow cytometry, the antibody serves as a crucial tool for studying HPK1’s role as a potential therapeutic target or biomarker in immune-related disorders. Its specificity for the phosphorylated T165 epitope ensures precise detection of activated MAP4K1 in complex biological samples.