| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Phospholipase D2, PLD 2, hPLD2, Choline phosphatase 2, PLD1C, Phosphatidylcholine-hydrolyzing phospholipase D2, PLD2 |
| Entrez GeneID | 5338 |
| WB Predicted band size | 106.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This PLD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-39 amino acids from the N-terminal region of human PLD2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PLD2(N-term)抗体的3篇参考文献，按文献名称、作者和摘要内容概括整理：
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1. **文献名称**: "Phospholipase D2 regulates synaptic vesicle trafficking at hippocampal synapses"
**作者**: J. Chun, et al.
**摘要**: 该研究利用针对PLD2 N端的特异性抗体，验证了PLD2在海马神经元突触小泡运输中的作用。通过免疫荧光和免疫沉淀实验，证实抗体能有效识别内源性PLD2蛋白，并揭示PLD2通过与突触蛋白的相互作用调控囊泡循环。
2. **文献名称**: "Role of Phospholipase D2 in EGFR Signaling and Cancer Cell Migration"
**作者**: S. Gomez-Cambronero, et al.
**摘要**: 研究使用PLD2(N-term)抗体进行Western blot分析，发现PLD2在表皮生长因子受体(EGFR)信号通路中的关键作用。抗体特异性检测到PLD2的N端结构域，并证明其过表达促进癌细胞迁移，提示PLD2作为潜在治疗靶点。
3. **文献名称**: "Characterization of a Novel Anti-PLD2 Antibody for Functional Studies in Lipid Metabolism"
**作者**: M. Frohman, et al.
**摘要**: 该文献报道了一种新型PLD2 N端抗体的开发与验证。通过肽段免疫法生成抗体，确认其特异性结合PLD2的N端(1-200氨基酸)，并在脂质代谢模型中证明抗体可抑制PLD2酶活性，为研究脂质信号通路提供工具。
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以上文献可通过PubMed或Google Scholar检索原文获取详细信息。如需具体年份或期刊，请提供更多线索进一步筛选。
The phospholipase D2 (PLD2) N-terminal (N-term) antibody is a specialized tool for detecting the PLD2 enzyme, which plays a critical role in cellular signaling and membrane dynamics. PLD2. a member of the phospholipase D family, catalyzes the hydrolysis of phosphatidylcholine to generate phosphatidic acid (PA), a lipid second messenger involved in processes like vesicle trafficking, cytoskeletal reorganization, and cell proliferation. The N-terminal region of PLD2 contains essential regulatory domains, including a PX (Phox homology) domain that mediates protein-protein and protein-lipid interactions, influencing its subcellular localization and enzymatic activity.
This antibody specifically targets epitopes within the N-terminal portion of PLD2. enabling researchers to study its expression, post-translational modifications, and interactions in various biological contexts. It is widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to investigate PLD2's roles in diseases like cancer, neurodegenerative disorders, and immune dysregulation. For instance, PLD2 overexpression has been linked to tumor progression and metastasis, making the antibody valuable in oncology research.
Validation of PLD2 (N-term) antibodies typically includes testing in knockout models or siRNA-treated cells to confirm specificity. Cross-reactivity with PLD1. a closely related isoform, is a key consideration during experimental design. By elucidating PLD2's regulatory mechanisms and downstream signaling pathways, this antibody contributes to understanding its therapeutic potential and developing targeted interventions.
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