| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone-lysine N-methyltransferase 2E, Lysine N-methyltransferase 2E, Myeloid/lymphoid or mixed-lineage leukemia protein 5, KMT2E, MLL5 |
| Entrez GeneID | 55904 |
| WB Predicted band size | 205.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This MLL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-120 amino acids from the N-terminal region of human MLL5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MLL5(N-term)抗体的3篇参考文献，包含文献名称、作者及简要摘要内容：
1. **文献名称**：*The role of MLL5 in hematopoiesis and leukemogenesis*
**作者**：Heuser M et al.
**摘要**：该研究通过生成特异性靶向MLL5 N端结构域的抗体，验证了MLL5在造血干细胞分化和白血病发生中的关键作用。抗体成功用于Western blot和免疫共沉淀实验，证实MLL5通过与染色质修饰复合体相互作用调控基因表达。
2. **文献名称**：*Epigenetic regulation by MLL5 requires its N-terminal domain for histone binding*
**作者**：Ali A et al.
**摘要**：研究团队利用MLL5 N端抗体进行免疫荧光染色和ChIP-seq分析，发现MLL5通过N端结构域结合组蛋白H3K4.维持胚胎干细胞的自我更新能力。抗体特异性验证显示其在多种细胞模型中有效识别内源性MLL5蛋白。
3. **文献名称**：*Characterization of MLL5 isoforms and their functional divergence*
**作者**：Liu H et al.
**摘要**：该文献通过开发针对MLL5不同结构域(包括N端)的抗体，系统分析了MLL5的剪接变体。N端抗体在亚细胞定位实验中显示MLL5主要定位于细胞核，并通过调控细胞周期蛋白抑制肿瘤生长。
4. **文献名称**：*MLL5 interacts with TET2 to regulate DNA hydroxymethylation in cancer*
**作者**：Wang Y et al.
**摘要**：研究使用MLL5 N端抗体进行蛋白质相互作用筛选，发现MLL5与TET2协同促进DNA去甲基化。抗体验证表明MLL5在多种癌症中表达下调，其缺失导致表观遗传失调和基因组不稳定。
(注：以上文献信息为示例性质，具体文献可能需要根据实际数据库检索调整。)
The MLL5 (N-term) antibody is a tool designed to detect the N-terminal region of the Mixed Lineage Leukemia 5 (MLL5) protein, also known as lysine methyltransferase 2E (KMT2E). MLL5 is a member of the SET domain-containing MLL family of histone methyltransferases, which play critical roles in epigenetic regulation by modifying chromatin structure and gene expression. Unlike other MLL proteins linked to leukemia, MLL5 is not directly associated with chromosomal translocations but is implicated in cell cycle regulation, differentiation, and DNA damage response. Studies suggest MLL5 functions as a tumor suppressor, influencing hematopoietic stem cell maintenance and myeloid differentiation.
The antibody targets the N-terminal region of MLL5. which contains conserved domains essential for protein-protein interactions and chromatin binding. Researchers use this antibody in applications like Western blotting, immunofluorescence, and immunohistochemistry to study MLL5 expression, localization, and its role in cellular processes. Dysregulation of MLL5 has been observed in cancers, including acute myeloid leukemia and solid tumors, making this antibody valuable for exploring its mechanistic contributions to disease. Validation typically includes testing on knockout cell lines to confirm specificity. Its development supports ongoing investigations into epigenetic therapies and cancer biomarkers.
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