Rabbit Polyclonal SLC2A8 Antibody

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     All lanes : Anti-SLC2A8 Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: 293 whole cell lysate Lane 3: human testis lysate Lane 4: Caco2 whole cell lysate Lane 5: mouse testis lysate Lane 6: rat testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 51 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Overlay histogram showing U-2 OS cells stained with P34184(green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34184,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

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     P34184 staining SLC2A8 in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,  paraffin-embedded sections).  Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6).  Samples were incubated with primary antibody (1/25) for 1 hours at 37°C.  A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.    

The SLC2A8 antibody is a research tool designed to detect and study the solute carrier family 2 member 8 (SLC2A8), also known as glucose transporter 8 (GLUT8). Encoded by the *SLC2A8* gene, GLUT8 is a facilitative transporter primarily involved in cellular glucose uptake and metabolism. It belongs to the GLUT family of transmembrane proteins, which regulate energy homeostasis by transporting hexoses across cell membranes. GLUT8 is structurally characterized by 12 transmembrane domains and intracellular localization, requiring trafficking via the Golgi apparatus or endosomal system for functional membrane integration.

Expressed in tissues such as the brain, testis, and adipose tissue, GLUT8 is implicated in insulin-regulated glucose metabolism, though its physiological role remains less defined compared to other GLUT isoforms. Studies suggest its involvement in metabolic disorders, neurodegenerative diseases, and cancer progression. For example, altered GLUT8 expression has been observed in certain cancers (e.g., breast, prostate) and under conditions of cellular stress or nutrient deprivation.

SLC2A8 antibodies are typically developed in hosts like rabbits or mice using immunogenic peptide sequences specific to GLUT8. They enable techniques such as Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression, localization, and interactions. Validated antibodies undergo specificity checks (e.g., knockout controls) to ensure reliability. Researchers use these tools to explore GLUT8's role in metabolic pathways, disease mechanisms, and therapeutic targeting, emphasizing its potential as a biomarker or drug target in metabolic and oncological research.