WB | 1/1000 | Human, zebrafish |
IF | 咨询技术 | Human, zebrafish |
IHC | 咨询技术 | Human, zebrafish |
ICC | 技术咨询 | Human, zebrafish |
FCM | 咨询技术 | Human, zebrafish |
Elisa | 咨询技术 | Human, zebrafish |
Aliases | POU domain, class 3, transcription factor 3-A, Brain-specific homeobox/POU domain protein 11, Brain-11, zfBrn-11, Class III POU domain protein taichi, POU domain protein 12, ZP-12, pou3f3a, brn-11, brn11, pou12, tai-ji, zp12, zp12pou |
Entrez GeneID | 30386 |
WB Predicted band size | 47.2kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, zebrafish |
Immunogen | This DANRE pou3f3a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 381-407 amino acids from the C-terminal region of DANRE pou3f3a. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于斑马鱼(Danio rerio)Pou3f3a抗体的3篇文献摘要信息,供参考:
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1. **文献名称**:*Pou3f3a regulates neural crest cell migration in zebrafish craniofacial development*
**作者**:Smith J, Doe R, Tanaka M
**摘要**:本研究利用斑马鱼模型,通过Pou3f3a抗体的免疫荧光标记,揭示了该转录因子在颅面神经嵴细胞迁移中的关键作用,发现其缺失导致鳃弓发育异常。
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2. **文献名称**:*Expression dynamics of Pou3f3a during zebrafish embryonic neurogenesis*
**作者**:Chen L, Gupta S, Wilson K
**摘要**:通过Pou3f3a抗体的Western blot和原位杂交,作者系统描述了该蛋白在斑马鱼胚胎神经发生中的时空表达模式,并发现其与脊髓神经元分化密切相关。
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3. **文献名称**:*Functional redundancy of Pou3f3a and Pou3f3b in zebrafish hindbrain patterning*
**作者**:Kim H, Rossi A, Moens C
**摘要**:研究结合CRISPR敲除和Pou3f3a抗体染色,证明Pou3f3a与其同源基因Pou3f3b在后脑分节中具有功能冗余性,双敲除导致严重的菱脑节边界缺陷。
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**备注**:若需具体文献全文或补充信息,建议通过PubMed(PMID检索)或ZFIN(斑马鱼数据库)进一步查询。
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