Rabbit Polyclonal POU2AF1(N-term) Antibody

The POU2AF1 (N-term) antibody is designed to target the N-terminal region of the POU2AF1 protein, a key transcriptional coactivator also known as OCA-B or BOB1. POU2AF1 plays a critical role in B-cell development and immune response regulation by binding to POU-domain transcription factors like OCT1 (POU2F1) or OCT2 (POU2F2). This interaction facilitates the activation of B-cell-specific genes, including immunoglobulin promoters, and is essential for germinal center formation and antibody production. The N-terminal domain of POU2AF1 is crucial for its interaction with OCT proteins and recruitment to target DNA sites. Antibodies targeting this region are widely used in research to study POU2AF1 expression, localization, and function in normal and pathological contexts, such as B-cell lymphomas. They are validated for applications like Western blotting, immunoprecipitation, and immunohistochemistry. Studies using these antibodies have highlighted POU2AF1's role in maintaining B-cell identity and its dysregulation in malignancies. For example, loss of POU2AF1 in mouse models leads to impaired B-cell maturation, underscoring its biological significance. The antibody's specificity for the N-terminal epitope ensures minimal cross-reactivity, making it a reliable tool for dissecting molecular mechanisms in immunology and cancer research.