| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Zinc finger protein 12, Gonadotropin-inducible ovary transcription repressor 3, GIOT-3, Zinc finger protein 325, Zinc finger protein KOX3, ZNF12, GIOT3, KOX3, ZNF325 |
| Entrez GeneID | 7559 |
| WB Predicted band size | 81.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ZNF12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-128 amino acids from the N-terminal region of human ZNF12. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ZNF12 (N-terminal)抗体的示例参考文献(注：因具体文献数据库访问限制，以下为示例性内容，建议通过PubMed或专业平台核实)：
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1. **文献名称**: "ZNF12 regulates early neural development through transcriptional repression"
**作者**: Smith A, et al.
**摘要**: 本研究利用ZNF12 (N-term)抗体进行免疫沉淀和染色质分析，发现ZNF12通过结合特定基因启动子抑制神经前体细胞分化相关基因的表达，在小鼠胚胎发育中起关键作用。
2. **文献名称**: "Characterization of zinc finger protein 12 interactions using N-terminal specific antibodies"
**作者**: Lee H, et al.
**摘要**: 通过ZNF12 (N-term)抗体的Western blot和免疫共沉淀实验，揭示了ZNF12与染色质重塑复合物成员HDAC1的相互作用，提示其在表观遗传调控中的功能。
3. **文献名称**: "Expression profiling of ZNF12 in human tissues and cancer cell lines"
**作者**: Gonzalez R, et al.
**摘要**: 使用ZNF12 N端特异性抗体进行组织芯片分析，发现ZNF12在多种癌组织中表达上调，可能作为癌症诊断的潜在生物标志物。
4. **文献名称**: "ZNF12 knockdown impairs cell cycle progression in vitro"
**作者**: Chen L, et al.
**摘要**: 通过siRNA敲低ZNF12并结合N-terminal抗体验证蛋白表达，发现ZNF12缺失导致细胞周期G1/S期阻滞，提示其在细胞增殖中的调控作用。
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建议通过PubMed、Google Scholar或Web of Science搜索关键词 **"ZNF12 antibody N-terminal"** 或 **"ZNF12 immunolocalization"** 获取最新文献。如需具体文献DOI或发表年份，请提供更多信息以便进一步筛选。
The ZNF12 (N-term) antibody is designed to target the N-terminal region of Zinc Finger Protein 12 (ZNF12), a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family. These proteins are characterized by tandem C₂H₂-type zinc finger motifs, which mediate sequence-specific DNA binding, and an N-terminal KRAB domain that often functions as a transcriptional repression module by recruiting chromatin-modifying complexes. ZNF12 is implicated in regulating gene expression, particularly in processes such as cell differentiation, proliferation, and apoptosis, though its precise biological roles remain under investigation.
The N-terminal region of ZNF12. recognized by this antibody, includes the KRAB domain critical for protein-protein interactions and transcriptional regulation. Studies using ZNF12 antibodies often focus on elucidating its expression patterns, subcellular localization (primarily nuclear), and involvement in signaling pathways or disease contexts, such as cancer or developmental disorders. The antibody is commonly employed in techniques like Western blotting, immunofluorescence, or chromatin immunoprecipitation (ChIP) to assess protein levels, interactions, or DNA-binding activity. Validation typically includes testing in ZNF12-knockout models or siRNA-treated cells to confirm specificity. Research utilizing this antibody contributes to understanding the role of KRAB-ZFPs in epigenetic regulation and their potential as therapeutic targets.
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